Polycarpine hydrochloride
Based on 1 Customer Validation
Polycarpine hydrochloride (1a) is a broad-spectrum Mpro inhibitor (IC50 = 30 nM) that can be isolated from the Polycarpa aurata and also serves as an anti-coronaviral agent. Polycarpine hydrochloride possesses antiviral and antifungal activities, with IC50 values of 30.0 nM and 0.12 μM against SARS-CoV-2 Mpro and PEDV Mpro, respectively.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 98.05%
- CAS. Nr.: 175669-29-1
- Formel: C22H26Cl2N6O2S2
- Molecular Weight:541.52
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Beschreibung
IC50 & Target
IC50: 30 nM (Mpro)[1].
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Vero C1008 | CC50 |
50.2 μM
Compound: 1a
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Cytotoxicity against African green monkey Vero E6 cells assessed as reduction of cell viability
Cytotoxicity against African green monkey Vero E6 cells assessed as reduction of cell viability
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[PMID: 38508325] |
Chemical Information
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CAS. Nr. 175669-29-1
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Appearance Solid
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Molecular Weight 541.52
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Formel C22H26Cl2N6O2S2
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Color Yellow to orange
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SMILES
COC1=CC=C(C=C1)C2=C(N(C(N)=N2)C)SSC3=C(N=C(N3C)N)C4=CC=C(C=C4)OC.Cl.Cl
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Structure Classification
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Initial Source
Polycarpa aurata
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 83.33 mg/mL (153.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Protokoll
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Reinheit & Dokumentation
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Data Sheet (277 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8467 mL | 9.2333 mL | 18.4665 mL | 46.1663 mL |
| 5 mM | 0.3693 mL | 1.8467 mL | 3.6933 mL | 9.2333 mL | |
| 10 mM | 0.1847 mL | 0.9233 mL | 1.8467 mL | 4.6166 mL | |
| 15 mM | 0.1231 mL | 0.6156 mL | 1.2311 mL | 3.0778 mL | |
| 20 mM | 0.0923 mL | 0.4617 mL | 0.9233 mL | 2.3083 mL | |
| 25 mM | 0.0739 mL | 0.3693 mL | 0.7387 mL | 1.8467 mL | |
| 30 mM | 0.0616 mL | 0.3078 mL | 0.6156 mL | 1.5389 mL | |
| 40 mM | 0.0462 mL | 0.2308 mL | 0.4617 mL | 1.1542 mL | |
| 50 mM | 0.0369 mL | 0.1847 mL | 0.3693 mL | 0.9233 mL | |
| 60 mM | 0.0308 mL | 0.1539 mL | 0.3078 mL | 0.7694 mL | |
| 80 mM | 0.0231 mL | 0.1154 mL | 0.2308 mL | 0.5771 mL | |
| 100 mM | 0.0185 mL | 0.0923 mL | 0.1847 mL | 0.4617 mL |
Polycarpine hydrochloride Related Classifications
HY-N12697A Related Classifications
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