1. Autophagy
  2. Autophagy
  3. Autophagy inducer 4

Autophagy inducer 4 

Cat. No.: HY-146087
Handling Instructions

Autophagy inducer 4 is a Magnolol-based Mannich base derivatives, which can be used as an anticancer agent. Autophagy inducer 4 suppresses cancer cells via inducing autophagy. Autophagy inducer 4 has 76-fold improvement in cytotoxicity against T47D cells compared with Magnolol. Autophagy inducer 4 also possesses suppressive effects on migration of T47D and Hela cancer cells.

For research use only. We do not sell to patients.

Autophagy inducer 4 Chemical Structure

Autophagy inducer 4 Chemical Structure

CAS No. : 2486455-03-0

Size Stock
100 mg   Get quote  
250 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Autophagy inducer 4 is a Magnolol-based Mannich base derivatives, which can be used as an anticancer agent. Autophagy inducer 4 suppresses cancer cells via inducing autophagy. Autophagy inducer 4 has 76-fold improvement in cytotoxicity against T47D cells compared with Magnolol. Autophagy inducer 4 also possesses suppressive effects on migration of T47D and Hela cancer cells[1].

IC50 & Target

IC50: 0.91 μM in T47D, 3.32 μM in MCF-7, 1.71 μM in Hela[1]

In Vitro

Autophagy inducer 4 (compound 3p) (0-10 μM; 72 hours) displays highly potent antiproliferative activity against T47D, MCF-7 and Hela cell lines[1].
Autophagy inducer 4 (40-80 μM; 0-36 hours) significantly increases GFP-LC3 protein puncta with both dose- and time-dependent manners in HEK293 cells[1].
Autophagy inducer 4 (0-80 μM; 0-36 hours) increases the transformation of LC3-I into LC3-II in a dose- and time-dependent manner[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line: T47D, MCF-7 and Hela cells[1]
Concentration: 0-10 μM
Incubation Time: 72 hours
Result: Displayed highly potent antiproliferative activity against T47D, MCF-7 and Hela cell lines with IC50 values of 0.91, 3.32 and 1.71 μM, respectively.

Cell Autophagy Assay

Cell Line: GFP-LC3-HEK293[1]
Concentration: 40, 60 and 80 μM
Incubation Time: 0, 12, 24 and 36 hours
Result: Significantly increased GFP-LC3 protein puncta with both dose- and time-dependent manners in HEK293 cells.

Western Blot Analysis

Cell Line: Hela, T47D and HEK293[1]
Concentration: 0, 5, 10 and 20 μM in T47D and Hela; 0, 40, 60 and 80 μM in HEK293
Incubation Time: 0, 12, 24 and 36 hours
Result: Increased the transformation of LC3-I into LC3-II in a dose-dependent manner, and enhanced the expression of LC3-II in a time-dependent manner.
Molecular Weight

531.64

Formula

C32H37NO6

CAS No.
SMILES

OC1=C(C2=CC(CC=C)=CC(C(N3CCOCC3)C4=CC(OC)=C(C(OC)=C4)OC)=C2O)C=C(CC=C)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Autophagy inducer 4
Cat. No.:
HY-146087
Quantity:
MCE Japan Authorized Agent: