Azalamellarin N
Based on 3 publication(s) in Google Scholar
Azalamellarin N is an inhibitor of pyroptosis and has different inhibitory effects on different pyroptosis inducers. Azalamellarin N inhibits pyroptosis by targeting molecules that act upstream of NLRP3 inflammasome activation, rather than directly targeting components of the NLRP3 inflammasome. Inhibitory potency against different pyroptosis inducers: Nigericin (HY-127019) > R837 (HY-B0180).
For research use only. We do not sell to patients.
- Purity : 99.58%
- CAS No.: 1809535-57-6
- Formula: C28H22N2O7
- Molecular Weight:498.48
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Azalamellarin N
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Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
0.0155 μM
Compound: 5
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Cytotoxicity against human A549 cells harboring wild type EGFR assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human A549 cells harboring wild type EGFR assessed as reduction in cell viability after 72 hrs by MTT assay
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[PMID: 33556869] |
| NCI-H1975 | IC50 |
0.0019 μM
Compound: 5
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Cytotoxicity against human NCI-H1975 cells harboring T790M/L858R mutant assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human NCI-H1975 cells harboring T790M/L858R mutant assessed as reduction in cell viability after 72 hrs by MTT assay
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[PMID: 33556869] |
In Vitro
Azalamellarin N (0.125-1 μM, pretreated 30 min) attenuates ATP- and nigericin-induced pyroptosis and activation of the NLRP3 inflammasome[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:THP-1, J774.1
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Concentration:0.125, 0.25, 0.5, 1 μM
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Incubation Time:pretreated 30 min
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Result:Strongly inhibited ATP- and nigericin-induced IL-1β release, but only modestly inhibited R837-induced IL-1β release.
Inhibited Nigericin-induced cleavage of caspase-1.
Suppressed ATP-induced oligomerization of ASC to a similar extent to MCC950.
Chemical Information
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CAS No. 1809535-57-6
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Appearance Solid
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Molecular Weight 498.48
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Formula C28H22N2O7
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Color White to off-white
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SMILES
OC1=CC(C2=C(C(C=C(OC)C(O)=C3)=C3C=C4)N4C5=C2C6=C(C=C(O)C(OC)=C6)NC5=O)=CC=C1OC
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Synonyms
AZL-N
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (3)
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Journal Impact Factor
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Most Recent
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J Extracell Vesicles
Chemoradiotherapy-Integrated Tumor Cell-Derived Microparticles Mediate Tumor Eradication in Malignant Pleural Effusion. [Abstract]2026 Apr;15(4):e70277. PMID: 41944389 -
J Colloid Interface Sci
Photoactive metal-covalent organic framework nanozymes with enhanced peroxidase-mimicking activity for eliminating drug-resistant bacterial infections. [Abstract]2025 Jun 12;699(Pt 1):138178. PMID: 40527142 -
Cent Eur J Immunol
Inhibition of XPOT promotes breast cancer cell pyroptosis to suppress cancer progression. [Abstract]2025;50(3):248-259. PMID: 41438360
Solvent & Solubility
In Vitro:
DMSO : ≥ 100 mg/mL (200.61 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Pyroptosis Solutions
Pyroptosis is a lytic inflammatory cell-death pathway executed by gasdermin pores, most classically through inflammasome-mediated activation of caspase-1, cleavage of gasdermin D, membrane pore formation, LDH release, and secretion of IL-1β and IL-18. The canonical pathway is commonly modeled by priming cells with an inflammatory signal such as LPS to induce pro-IL-1β and inflammasome components, followed by an activation signal such as ATP or nigericin to activate NLRP3, ASC speck formation, caspase-1 cleavage, GSDMD cleavage, cytokine release, and pyroptotic membrane rupture. The non-canonical pathway is triggered when cytosolic LPS activates mouse caspase-11 or human caspase-4/5, leading to GSDMD cleavage and pyroptosis, and this can secondarily activate NLRP3-dependent IL-1β release. Pyroptosis is linked to inflammatory injury, infection, cancer, liver disease, ocular disease, placental inflammation, and other disease phenotypes, but unresolved questions include which gasdermin fam
Purity & Documentation
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Data Sheet (284 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0061 mL | 10.0305 mL | 20.0610 mL | 50.1525 mL |
| 5 mM | 0.4012 mL | 2.0061 mL | 4.0122 mL | 10.0305 mL | |
| 10 mM | 0.2006 mL | 1.0030 mL | 2.0061 mL | 5.0152 mL | |
| 15 mM | 0.1337 mL | 0.6687 mL | 1.3374 mL | 3.3435 mL | |
| 20 mM | 0.1003 mL | 0.5015 mL | 1.0030 mL | 2.5076 mL | |
| 25 mM | 0.0802 mL | 0.4012 mL | 0.8024 mL | 2.0061 mL | |
| 30 mM | 0.0669 mL | 0.3343 mL | 0.6687 mL | 1.6717 mL | |
| 40 mM | 0.0502 mL | 0.2508 mL | 0.5015 mL | 1.2538 mL | |
| 50 mM | 0.0401 mL | 0.2006 mL | 0.4012 mL | 1.0030 mL | |
| 60 mM | 0.0334 mL | 0.1672 mL | 0.3343 mL | 0.8359 mL | |
| 80 mM | 0.0251 mL | 0.1254 mL | 0.2508 mL | 0.6269 mL | |
| 100 mM | 0.0201 mL | 0.1003 mL | 0.2006 mL | 0.5015 mL |
Azalamellarin N Related Classifications
HY-162150 Related Classifications
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