Biotin-11-dATP
Biotin-11-dATP is a biotinylated deoxyadenosine triphosphate analog. Biotin-11-dATP serves as a substrate for Klenow fragment fill-in reactions to biotinylate linearized plasmid DNA.
For research use only. We do not sell to patients.
- CAS No.: 2567941-51-7
- Formula: C30H45N8O15P3S
- Molecular Weight:882.71
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Description
In Vitro
Biotin-11-dATP is incorporated into Adcy1-Adcy6 cDNA probes by nick translation, yielding 200-400 bp labeled products[2].
Biotin-11-dATP-labeled HD cosmid probe detects single integration sites by FISH in transgenic FVB/N mouse metaphase spreads, including chromosome 1 D-E in YGA2 line 30 and chromosome 2 C3-D in 353G6 line B60[3].
Biotin-11-dATP was incorporated by nick translation into approximately 40 kb GPV and GPIX cosmid clones, yielding 200-400 bp labeled probes[5].
Biotin-11-dATP-labeled GPV and GPIX cosmid probes produced FISH signals at chromosome 3q29 (88/119 spreads) and 3q21 (15/82 spreads), respectively, in metaphase chromosome preparations from normal human male lymphocyte cultures[5].
Biotin-11-dATP-labeled C0t-DNA and DOP-PCR probes hybridize broadly to heterochromatic chromosome arms of M. scutellaris, supporting enrichment of repetitive DNA in heterochromatin[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2567941-51-7
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Molecular Weight 882.71
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Formula C30H45N8O15P3S
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SMILES
O[C@H]1C[C@H](N(C2=NC=NC(N)=C23)C=C3C#CCNC(CCCCCNC(CCCC[C@H]4[C@]5([H])[C@](NC(N5)=O)([H])CS4)=O)=O)O[C@@H]1COP(OP(OP(O)(O)=O)(O)=O)(O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)