Biotin-C2-maleimide
Based on 1 Customer Validation
Biotin-C2-maleimide is an antibody conjugate reagent, can bind to many biomolecules without significantly changing the biological activity of the target molecule.
For research use only. We do not sell to patients.
- Purity: 99.48%
- CAS No.: 139554-72-6
- Formula: C16H22N4O4S
- Molecular Weight:366.44
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
Biotin-C2-maleimide can act bioconjugation with 5 eq. SUMO-1 probes[1].
1. Biotin C2 maleimide (ATT Bioquest, dissolved in DMSO) is performed in Tris buffer (50 mM Tris, 300 mM NaCl; pH 7.0) after 15 min reduction with Tris (2-carboxyethyl)phosphine (TCEP, 120 μM) at room temperature.
2. After 1 h incubation, the unreacted Biotin is separated by Ni-NTA affinity chromatography followed by dialysis against PBS buffer (pH 7.4).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 139554-72-6
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Appearance Solid
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Molecular Weight 366.44
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Formula C16H22N4O4S
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Color White to off-white
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SMILES
O=C(NCCN1C(C=CC1=O)=O)CCCC[C@@H]2SC[C@]([C@]2([H])N3)([H])NC3=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 125 mg/mL (341.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7290 mL | 13.6448 mL | 27.2896 mL | 68.2240 mL |
| 5 mM | 0.5458 mL | 2.7290 mL | 5.4579 mL | 13.6448 mL | |
| 10 mM | 0.2729 mL | 1.3645 mL | 2.7290 mL | 6.8224 mL | |
| 15 mM | 0.1819 mL | 0.9097 mL | 1.8193 mL | 4.5483 mL | |
| 20 mM | 0.1364 mL | 0.6822 mL | 1.3645 mL | 3.4112 mL | |
| 25 mM | 0.1092 mL | 0.5458 mL | 1.0916 mL | 2.7290 mL | |
| 30 mM | 0.0910 mL | 0.4548 mL | 0.9097 mL | 2.2741 mL | |
| 40 mM | 0.0682 mL | 0.3411 mL | 0.6822 mL | 1.7056 mL | |
| 50 mM | 0.0546 mL | 0.2729 mL | 0.5458 mL | 1.3645 mL | |
| 60 mM | 0.0455 mL | 0.2274 mL | 0.4548 mL | 1.1371 mL | |
| 80 mM | 0.0341 mL | 0.1706 mL | 0.3411 mL | 0.8528 mL | |
| 100 mM | 0.0273 mL | 0.1364 mL | 0.2729 mL | 0.6822 mL |
Biotin-C2-maleimide Related Classifications
HY-D1546 Related Classifications
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