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Cat. No.: HY-E72123

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BirA biotin ligase

BirA biotin ligase is a biotin ligase that targets the Avi tag. BirA biotin ligase catalyzes the in vitro biotinylation of Avi-tagged substrates by site-specifically conjugating biotin to the lysine side chain within the Avi tag. BirA biotin ligase retains catalytic activity in thioredoxin and MBP fusion forms and can be used directly for biotinylation; this enzyme is suitable for in vitro protein biotinylation labeling studies.

For research use only. We do not sell to patients.

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (271 KB)

BirA biotin ligase Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

In Vitro

BirA biotin ligase (30 μM Avi-tagged thioredoxin; 4 h) has high activity in vitro, and at a 1:100 enzyme:substrate molar ratio at room temperature, it can completely biotinylate 30 μM Avi-tagged thioredoxin within 4 h^[1].
Recombinant BirA biotin ligase (1 mM IPTG (HY-15921); 22 h) can be produced in BL21 (DE3) as a thioredoxin or MBP fusion and released by TEV cleavage, yielding 24.7 and 27.6 mg of purified ligase per liter of culture, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

SMILES

[BirA biotin ligase]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Li Y, et al. Expression and purification of E. coli BirA biotin ligase for in vitro biotinylation. Protein expression and purification. 2012 Mar;82(1):162-7.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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