Boc-L-Cys(Propargyl)-OH (DCHA)
Boc-L-Cys(Propargyl)-OH (DCHA) is a click chemistry reagent. Boc-L-Cys(Propargyl)-OH (DCHA) can be used for the research of various biochemical.
For research use only. We do not sell to patients.
- Formula: C23H40N2O4S
- Molecular Weight:440.64
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Chemical Information
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Molecular Weight 440.64
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Formula C23H40N2O4S
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SMILES
CC(C)(C)OC(N[C@H](C(O)=O)CSCC#C)=O.C1(CCCCC1)NC2CCCCC2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
References
[1]. Mauro Lo Conte, et al. Photoinduced addition of glycosyl thiols to alkynyl peptides: use of free-radical thiol-yne coupling for post-translational double-glycosylation of peptides. [Content Brief]
[2]. Charlotte Collet, et al. Development of 6-[(18) F]fluoro-carbohydrate-based prosthetic groups and their conjugation to peptides via click chemistry. J Labelled Comp Radiopharm. 2016 Feb;59(2):54-62. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Boc-L-Cys(Propargyl)-OH (DCHA) Related Classifications
HY-151652A Related Classifications
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