BOC-FlFlF
Based on 1 Customer Validation
BOC-FlFlF (Boc-Phe-dLeu-Phe-dLeu-Phe) is a selective FPR1 antagonist. Boc-FlFlF has an apparent dissociation constant (KD) of 230 nM as determined by the intracellular calcium mobilization assay. Boc-FlFlF can be used for the study of inflammation.
For research use only. We do not sell to patients.
- Purity: 98.31%
- CAS No.: 66556-73-8
- Formula: C44H59N5O8
- Molecular Weight:785.97
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Storage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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FPR1 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HL-60 | IC50 |
2 μM
Compound: 1
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Antagonist activity at formyl peptide receptor in human HL60 cells assessed as inhibition of fMLP-induced calcium mobilization incubated for 5 mins prior to fMLP challenge by Fura-2 method
Antagonist activity at formyl peptide receptor in human HL60 cells assessed as inhibition of fMLP-induced calcium mobilization incubated for 5 mins prior to fMLP challenge by Fura-2 method
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[PMID: 25035262] |
Boc-FlFlF (1 μM) exerts a selective antagonistic effect on FPR1 and does not inhibit the increase in intracellular calcium concentration induced by the FPR2-selective agonist MMK-1[1].
Boc-FlFlF (1 μM) has an apparent dissociation constant (KD) of 230 nM as determined by the intracellular calcium mobilization assay[1].
Boc-FlFlF (0.0001-100 µM, 5 min pre-treatment) inhibits fMLF (FPR agonist)-induced superoxide production in neutrophils in a concentration-dependent manner with an EC50of 0.25 µM [2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 66556-73-8
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Appearance Solid
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Molecular Weight 785.97
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Formula C44H59N5O8
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Color White to off-white
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Synonyms
BOC2; Boc-Phe-dLeu-Phe-dLeu-Phe
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Sequence
Boc-Phe-dLeu-Phe-dLeu-Phe
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Sequence Shortening
Boc-F-dLeu-F-dLeu-F
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Solvent & Solubility
DMSO : 100 mg/mL (127.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Hayashi R, et al. Development of potent antagonists for formyl peptide receptor 1 based on Boc-Phe-D-Leu-Phe-D-Leu-Phe-OH. Bioorg Med Chem. 2014 Aug 1;22(15):3824-8. [Content Brief]
[2]. Stenfeldt AL, et al. Cyclosporin H, Boc-MLF and Boc-FLFLF are antagonists that preferentially inhibit activity triggered through the formyl peptide receptor. Inflammation. 2007 Dec;30(6):224-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2723 mL | 6.3616 mL | 12.7231 mL | 31.8078 mL |
| 5 mM | 0.2545 mL | 1.2723 mL | 2.5446 mL | 6.3616 mL | |
| 10 mM | 0.1272 mL | 0.6362 mL | 1.2723 mL | 3.1808 mL | |
| 15 mM | 0.0848 mL | 0.4241 mL | 0.8482 mL | 2.1205 mL | |
| 20 mM | 0.0636 mL | 0.3181 mL | 0.6362 mL | 1.5904 mL | |
| 25 mM | 0.0509 mL | 0.2545 mL | 0.5089 mL | 1.2723 mL | |
| 30 mM | 0.0424 mL | 0.2121 mL | 0.4241 mL | 1.0603 mL | |
| 40 mM | 0.0318 mL | 0.1590 mL | 0.3181 mL | 0.7952 mL | |
| 50 mM | 0.0254 mL | 0.1272 mL | 0.2545 mL | 0.6362 mL | |
| 60 mM | 0.0212 mL | 0.1060 mL | 0.2121 mL | 0.5301 mL | |
| 80 mM | 0.0159 mL | 0.0795 mL | 0.1590 mL | 0.3976 mL | |
| 100 mM | 0.0127 mL | 0.0636 mL | 0.1272 mL | 0.3181 mL |
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