BODIPY TR methyl ester

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Vital staining procedures^[1]:
1. Dissolve BODIPY TR methyl ester in anhydrous dimethyl sulfoxide (DMSO) to prepare a 5 mM stock solution.
2. Dilute the DMSO stock solution in embryo rearing medium (ERM) buffered with 5 mM HEPES (pH 7.2) to prepare a BODIPY TR methyl ester labeling solution with a final concentration of 100 μM and a DMSO volume fraction of 2%.
3. Stain the embryos in 100 μM BODIPY TR methyl ester solution for 1 h, then wash them three times successively in HEPES-buffered ERM.
4. Mount the embryos in an open-faced chamber for imaging.
5. For embryos used in fixation experiments, fix them in HEPES-buffered ERM containing 4% paraformaldehyde at 4°C for 1 h, then wash with ERM and mount for confocal imaging.
6. Analyze the sample under a confocal microscope (Ex = 568 nm, Em = 625 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.