c-Fms-IN-10
Based on 1 Customer Validation
c-Fms-IN-10 is the derivative of thieno [3,2-d] pyrimidine, an kinase inhibitor of FMS (Colony stimulating factor-1 receptor, CSF-1R) with IC50 of 2 nM. c-Fms-IN-10 has anti-tumor activity.
For research use only. We do not sell to patients.
- Purity: 98.63%
- CAS No.: 1527517-50-5
- Formula: C22H19N7OS
- Molecular Weight:429.50
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Bioactive Compound Library Max
HY-L181
-
Bioactive Compound Library
HY-L001
-
Immunology/Inflammation Compound Library
HY-L007
-
Kinase Inhibitor Library
HY-L009
-
Protein Tyrosine Kinase Compound Library
HY-L016
-
Anti-Cancer Compound Library
HY-L025
-
Targeted Diversity Library
HY-L099
-
Membrane Protein-targeted Compound Library
HY-L149
-
Cytokine Inhibitors Library
HY-L161
-
Macrophage Related Compound Library
HY-L174
-
MCE Bioactive Compound Library
HY-L001V
-
Anti-Brain Cancer Compound Library
HY-L188
-
Protein Kinase Compound Library
HY-L196
-
Cell Proliferation Compound Library
HY-L201
-
High-Throughput Bioactive Compound Library
HY-L205
-
Posttranslational Modification Library
HY-L226
Biological Activity
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| NFS-60 | GI50 |
80 nM
Compound: 25
|
Growth inhibition of mouse NFS60 cells after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay
Growth inhibition of mouse NFS60 cells after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay
|
[PMID: 30522957] |
| NFS-60 | GI50 |
80 nM
Compound: A21
|
Growth inhibition of mouse NFS-60 cells
Growth inhibition of mouse NFS-60 cells
|
[PMID: 36335744] |
| Sf21 | IC50 |
145 nM
Compound: 25
|
Inhibition of recombinant human N-terminal GST-tagged FGFR1 (456 to 765 residues) expressed in baculovirus infected Sf21 insect cells using IGF-IRtide (12-527) as substrate in presence of [gamma-33P]-ATP or [gamma-32P]-ATP
Inhibition of recombinant human N-terminal GST-tagged FGFR1 (456 to 765 residues) expressed in baculovirus infected Sf21 insect cells using IGF-IRtide (12-527) as substrate in presence of [gamma-33P]-ATP or [gamma-32P]-ATP
|
[PMID: 30522957] |
| Sf21 | IC50 |
2 nM
Compound: 25
|
Antagonist activity at recombinant human N-terminal His6-tagged FMS (538 to end residues) expressed in baculovirus infected Sf21 insect cells using IGF-R1tide as substrate in presence of [gamma-33P]-ATP or [gamma-32P]-ATP
Antagonist activity at recombinant human N-terminal His6-tagged FMS (538 to end residues) expressed in baculovirus infected Sf21 insect cells using IGF-R1tide as substrate in presence of [gamma-33P]-ATP or [gamma-32P]-ATP
|
[PMID: 30522957] |
| Sf21 | IC50 |
208 nM
Compound: 25
|
Inhibition of recombinant human N-terminal His6-tagged Tie2 (771 to end residues) expressed in baculovirus infected Sf21 insect cells using Poly (Glu4-Tyr) (4:1) as substrate in presence of [gamma-33P]-ATP or [gamma-32P]-ATP
Inhibition of recombinant human N-terminal His6-tagged Tie2 (771 to end residues) expressed in baculovirus infected Sf21 insect cells using Poly (Glu4-Tyr) (4:1) as substrate in presence of [gamma-33P]-ATP or [gamma-32P]-ATP
|
[PMID: 30522957] |
| Sf21 | IC50 |
258 nM
Compound: 25
|
Inhibition of recombinant human N-terminal GST-tagged MELK (1 to 340 residues) expressed in baculovirus infected Sf21 insect cells using KKLNRTLSFAEPG as substrate in presence of [gamma-33P]-ATP or [gamma-32P]-ATP
Inhibition of recombinant human N-terminal GST-tagged MELK (1 to 340 residues) expressed in baculovirus infected Sf21 insect cells using KKLNRTLSFAEPG as substrate in presence of [gamma-33P]-ATP or [gamma-32P]-ATP
|
[PMID: 30522957] |
| Sf21 | IC50 |
527 nM
Compound: 25
|
Inhibition of recombinant human N-terminal His6-tagged KDR (790 to end residues) expressed in baculovirus infected Sf21 insect cells using MBP as substrate in presence of [gamma-33P]-ATP or [gamma-32P]-ATP
Inhibition of recombinant human N-terminal His6-tagged KDR (790 to end residues) expressed in baculovirus infected Sf21 insect cells using MBP as substrate in presence of [gamma-33P]-ATP or [gamma-32P]-ATP
|
[PMID: 30522957] |
| Sf21 | IC50 |
64 nM
Compound: 25
|
Inhibition of recombinant human N-terminal His6-tagged DDR2 (467 to end residues) expressed in baculovirus infected Sf21 insect cells using Axltide as substrate in presence of [gamma-33P]-ATP or [gamma-32P]-ATP
Inhibition of recombinant human N-terminal His6-tagged DDR2 (467 to end residues) expressed in baculovirus infected Sf21 insect cells using Axltide as substrate in presence of [gamma-33P]-ATP or [gamma-32P]-ATP
|
[PMID: 30522957] |
| Sf21 | IC50 |
80 nM
Compound: 25
|
Inhibition of recombinant human N-terminal GST-tagged c-RAF Y340D/Y341D double mutant (306 to 648 residues) expressed in baculovirus infected Sf21 insect cells using MEK1(K97R) as substrate in presence of [gamma-33P]-ATP or [gamma-32P]-ATP
Inhibition of recombinant human N-terminal GST-tagged c-RAF Y340D/Y341D double mutant (306 to 648 residues) expressed in baculovirus infected Sf21 insect cells using MEK1(K97R) as substrate in presence of [gamma-33P]-ATP or [gamma-32P]-ATP
|
[PMID: 30522957] |
Chemical Information
-
CAS No. 1527517-50-5
-
Appearance Solid
-
Molecular Weight 429.50
-
Formula C22H19N7OS
-
Color Light yellow to yellow
-
SMILES
NC1=NC=NC2=C1SC=C2C(NC3=C(C(C)=NN4CC5=CC=CC(C)=N5)C4=CC=C3)=O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 10 mg/mL (23.28 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 1 mg/mL (2.33 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 1 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 1 mg/mL (2.33 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 1 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (271 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3283 mL | 11.6414 mL | 23.2829 mL | 58.2072 mL |
| 5 mM | 0.4657 mL | 2.3283 mL | 4.6566 mL | 11.6414 mL | |
| 10 mM | 0.2328 mL | 1.1641 mL | 2.3283 mL | 5.8207 mL | |
| 15 mM | 0.1552 mL | 0.7761 mL | 1.5522 mL | 3.8805 mL | |
| 20 mM | 0.1164 mL | 0.5821 mL | 1.1641 mL | 2.9104 mL |
c-Fms-IN-10 Related Classifications
HY-126297 Related Classifications
Powered by Bioz