C12-NBD-ceramide
Based on 1 Customer Validation
C12-NBD-ceramide is a fluorescent analogue of ceramide, it can be used as a substrate in ceramidase assays.
For research use only. We do not sell to patients.
- Purity : 99.67%
- CAS No.: 202850-01-9
- Formula: C36H61N5O6
- Molecular Weight:659.90
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
C12-NBD-ceramide is used as a fluorescent nCDase substrate and reaction products will be separated by reverse-phase HPLC[2]:
1. 20 μM substrate, 1 nM nCDase in 75 mM NaCl, 25 mM HEPES (pH 8.0), and 0.4% Triton X-100, for 2 h at 37°C in a final volume of 100 μL.
2. Extract samples with 1:1 chloroform-methanol dries under nitrogen gas, and resuspends in 60 μL of HPLC mobile phase B. Separats reaction products by reversed-phase HPLC using a column(3 μm particle).
3. Mobile phase A contains 0.2% formic acid and 1 mM ammonium formate in HPLC-grade water. Mobile phase B contains 0.2% formic acid (IL) and 1 mM ammonium formate in HPLC-grade methanol.
4. FRET-based assay using 20 μM substrate ES.173.cds, 50 ng of nCDase, 75 mM NaCl, 15 mM sodium phosphate (pH 7.4), and 0.3% Triton X-100 for 3 h in 96 black well plates.
5. Measure resorufin fluorescence (Ex=347 nm, Em=430 and 530 nm).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Emission (Em)
538
Chemical Information
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CAS No. 202850-01-9
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Appearance Crystal
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Molecular Weight 659.90
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Formula C36H61N5O6
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Color Brown to black
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SMILES
CCCCCCCCCCCCC/C=C/[C@@H](O)[C@H](CO)NC(CCCCCCCCCCCNC1=CC=C([N+]([O-])=O)C2=NON=C12)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (151.54 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Tani M, et al. Specific and sensitive assay for alkaline and neutral ceramidases involving C12-NBD-ceramide. J Biochem. 1999 Apr;125(4):746-9. [Content Brief]
[2]. Otsuka Y, et al. Identification of Small-Molecule Inhibitors of Neutral Ceramidase (nCDase) via Target-Based High-Throughput Screening. SLAS Discov. 2021 Jan;26(1):113-121. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5154 mL | 7.5769 mL | 15.1538 mL | 37.8845 mL |
| 5 mM | 0.3031 mL | 1.5154 mL | 3.0308 mL | 7.5769 mL | |
| 10 mM | 0.1515 mL | 0.7577 mL | 1.5154 mL | 3.7885 mL | |
| 15 mM | 0.1010 mL | 0.5051 mL | 1.0103 mL | 2.5256 mL | |
| 20 mM | 0.0758 mL | 0.3788 mL | 0.7577 mL | 1.8942 mL | |
| 25 mM | 0.0606 mL | 0.3031 mL | 0.6062 mL | 1.5154 mL | |
| 30 mM | 0.0505 mL | 0.2526 mL | 0.5051 mL | 1.2628 mL | |
| 40 mM | 0.0379 mL | 0.1894 mL | 0.3788 mL | 0.9471 mL | |
| 50 mM | 0.0303 mL | 0.1515 mL | 0.3031 mL | 0.7577 mL | |
| 60 mM | 0.0253 mL | 0.1263 mL | 0.2526 mL | 0.6314 mL | |
| 80 mM | 0.0189 mL | 0.0947 mL | 0.1894 mL | 0.4736 mL | |
| 100 mM | 0.0152 mL | 0.0758 mL | 0.1515 mL | 0.3788 mL |
C12-NBD-ceramide Related Classifications
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