Azide-phenylalanine hydrochloride
Azide-phenylalanine hydrochloride is a phenylalanine derivative. Azide-phenylalanine (hydrochloride) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
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- CAS No.: 1409940-46-0
- Formule: C9H11ClN4O2
- Masse moléculaire:242.66
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Stockage:
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Activité biologique
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Chemical Information
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Activité biologique
Description
In Vitro
Azide-phenylalanine hydrochloride (Compound 8) is a phenylalanine derivative[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1409940-46-0
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Masse moléculaire 242.66
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Formule C9H11ClN4O2
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SMILES
N[C@H](C(O)=O)CC1=CC=CC(N=[N+]=[N-])=C1.Cl
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Synonyms
UAA crosslinker 2
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Azide-phenylalanine hydrochloride Related Classifications
HY-103700A Related Classifications
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