Biotin-14-dATP
Biotin-14-dATP is a deoxyadenosine triphosphate analog modified by Biotin (HY-B0511), serving as a DNA labeling reagent that acts on E. coli DNA polymerase I. When incorporated into DNA probes, Biotin-14-dATP undergoes specific hybridization with complementary target DNA sequences, and its biotin group can bind to streptavidin-alkaline phosphatase conjugates, enabling colorimetric detection.
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- CAS No.: 106519-34-0
- Formule: C32H54N9O15P3S
- Masse moléculaire:929.81
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
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Help & FAQs
Activité biologique
Description
IC50 & Target
[1]|
DNA Polymerase |
In Vitro
Biotin-14-dATP serves as a substrate for E. coli DNA polymerase I in nick translation reactions involving 5.4 Kb plasmid DNA; after incorporation into DNA strands, it enables visual capture and detection of nucleic acid probes via the specific interaction between biotin and streptavidin[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
Chemical Information
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CAS No. 106519-34-0
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Masse moléculaire 929.81
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Formule C32H54N9O15P3S
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SMILES
O=C(N1)N[C@](CS2)([H])[C@@]1([H])[C@@H]2CCCCC(NCCCCCC(NCCCCCCNC3=NC=NC4=C3N=CN4[C@H]5C[C@H](O)[C@@H](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)O5)=O)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Biotin-14-dATP Related Classifications
HY-188030 Related Classifications
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