Cyanine5.5 amine
Based on 1 publication(s) in Google Scholar
Cyanine5.5 amine (Cy 5.5 amine), a Cy5.5 Analogue, is a near-infrared (NIR) fluorescent dye (Ex=648 nm, Em=710 nm). Cyanine5.5 amine can be used in the preparation of Cy5.5-labeled nanoparticles, which can be tracked and imaged with low fluorescence background using confocal microscopy.
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- Pureté : 98.95%
- CAS No.: 2097714-45-7
- Formule: C46H58Cl2N4O
- Masse moléculaire:753.88
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Stockage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Cyanine5.5 amine
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Customer Validation & Images
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Cell Imaging/Staining
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In Vivo Imaging
Activité biologique
Description
In Vivo
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[2].
1. Freshly prepared suspensions of Cy5.5-PLGA (resuspended in 0.5 ml 1% poloxamer 188, vortexed gently and left for 30 min at ambient temperature before use) is administrated intravenously (0.5 mL).
2. Anesthetize the rats, protect the eye from drying out.
3. Fix the rats under a confocal scanning microscope with the eye positioned in working distance underneath the objective lens, and a cannula is inserted into the tail vein.
4. Observe the fluorescence in the retina, and capture the images at different time points (0, 1, 3, 5, 15, 30, 60, 90 min).
Note: The rats are kept on the heating plate during all the in vivo imaging process.
5. After in vivo real-time imaging, rats were euthanized with an overdose of aforementioned anesthetic and the eyeballs were enucleated and placed into cooled HEPES buffered solution (135 mM NaCl, 5 mM NaOH, 2.5 mM KCl, 7 mM MgCl2, 10 mM HEPES, 10 mM glucose; pH7.4).
6. Remove the anterior segment of eye and vitreous body, separate whole retina carefully, flat on the modified culture plate.
7. Incubate the whole mounts wit0.1 mg/mL Hoechst 33342 (HY-15559) in HEPES solution for 20 min for nuclei staining.
8. Fix flat mount retina with 4% paraformaldehyde solution for 20 min and wash with HEPES solution.
9. Capture the images immediately with microscope after preparation of retinal flat mount.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Emission (Em)
710
Excitation (Ex)
680
Chemical Information
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CAS No. 2097714-45-7
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Appearance Solid
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Masse moléculaire 753.88
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Formule C46H58Cl2N4O
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Color Purple to purplish red
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SMILES
O=C(CCCCCN1C2=C(C(C)(C)/C1=C\C=C\C=C\C3=[N+](C)C4=C(C3(C)C)C5=C(C=CC=C5)C=C4)C6=C(C=CC=C6)C=C2)NCCCCCCN.[Cl-].Cl
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Synonyms
Cy 5.5 amine; Lumiprobe Cy 5.5 amine
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Mater Today Bio
Immuno-protective cationic ginsenoside Rb1 vesicles for macrophage-mediated targeted therapy of inflammatory diseases. [Abstract]2025 Aug 12:34:102195. PMID: 40893350
Cyanine5.5 amine purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Aug 12:34:102195. [Abstract]
The materials were labeled with Cy5.5 and co-cultured with Raw264.7 macrophage cells. Cellular uptake was observed using confocal microscopy.
Cyanine5.5 amine purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Aug 12:34:102195. [Abstract]
Distribution of fluorescence from Cy5.5-labeled nanoparticles in BALB/c mice at 1or 4 h post-injection.
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (132.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Seungho Lim, et al. Intracellular Uptake Mechanism of Bioorthogonally Conjugated Nanoparticles on Metabolically Engineered Mesenchymal Stem Cells. Bioconjug Chem. 2021 Jan 20;32(1):199-214. [Content Brief]
[2]. Enqi Zhang, et al. Release kinetics of fluorescent dyes from PLGA nanoparticles in retinal blood vessels: In vivo monitoring and ex vivo localization. Eur J Pharm Biopharm. 2020 May;150:131-142. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3265 mL | 6.6324 mL | 13.2647 mL | 33.1618 mL |
| 5 mM | 0.2653 mL | 1.3265 mL | 2.6529 mL | 6.6324 mL | |
| 10 mM | 0.1326 mL | 0.6632 mL | 1.3265 mL | 3.3162 mL | |
| 15 mM | 0.0884 mL | 0.4422 mL | 0.8843 mL | 2.2108 mL | |
| 20 mM | 0.0663 mL | 0.3316 mL | 0.6632 mL | 1.6581 mL | |
| 25 mM | 0.0531 mL | 0.2653 mL | 0.5306 mL | 1.3265 mL | |
| 30 mM | 0.0442 mL | 0.2211 mL | 0.4422 mL | 1.1054 mL | |
| 40 mM | 0.0332 mL | 0.1658 mL | 0.3316 mL | 0.8290 mL | |
| 50 mM | 0.0265 mL | 0.1326 mL | 0.2653 mL | 0.6632 mL | |
| 60 mM | 0.0221 mL | 0.1105 mL | 0.2211 mL | 0.5527 mL | |
| 80 mM | 0.0166 mL | 0.0829 mL | 0.1658 mL | 0.4145 mL | |
| 100 mM | 0.0133 mL | 0.0663 mL | 0.1326 mL | 0.3316 mL |
Cyanine5.5 amine Related Classifications
HY-D1540 Related Classifications
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