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Cat. No.: HY-D2282

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DFHBI-BI

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DFHBI-BI is a mimic of the green fluorescence protein chromophore. DFHBI-BI can be bound and activated by Broccoli ( a fluorogenic RNA aptamer) with higher affinity. DFHBI-BI prevents thermal unfolding of Broccoli at 37°C in mammalian cells, subsequently forming more fluorescent Broccoli-BI complexes. The fluorescence wavelength is 559/635 nm.

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Pureté: 99.12%
CAS No.: 2561482-42-4
Formule: C₁₉H₁₄F₂N₄O₂
Masse moléculaire:368.34

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

DFHBI-BI Chemical Structure

CAS No.: 2561482-42-4

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- In Vitro
Chemical Information
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
Bioz

Activité biologique

Description

In Vitro

DFHBI-BI staining the cells with 10 µM for 2 hours, the fluorescence intensity of the cells was low. DFHBI-BI was applied to HEK293T cells expressing Broccoli circular RNA, and an increase in fluorescence intensity was observed^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 2561482-42-4
Appearance Solid
Masse moléculaire 368.34
Formule C₁₉H₁₄F₂N₄O₂
Color brownish yellow
SMILES

CC(N1CC2=C3C(N=CN3)=CC=C2)=N/C(C1=O)=C\C4=CC(F)=C(O)C(F)=C4
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (268 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Xing Li, et al. Fluorophore-Promoted RNA Folding and Photostability Enables Imaging of Single Broccoli-Tagged mRNAs in Live Mammalian Cells. Angew Chem Int Ed Engl. 2020 Mar 9;59(11):4511-4518. [Content Brief]

Calculators

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

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Concentration _(final) Concentration _(final)

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

Minimum order quantity

100 mg

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