KMG-104
Based on 1 Customer Validation
KMG-104 is a cell-impermeant highly selective fluorescent Mg2+ probe. KMG-104 has been used widely and revealed Mg2+ mobilization in cytoplasm in various types of cells.
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- Pureté: 99.12%
- CAS No.: 852057-94-4
- Formule: C23H11F2NO6
- Masse moléculaire:435.33
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Stockage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
KMG-104 can be introduced into cells by methods such as patch clamp and microinjection.
KMG-104 is suitable for confocal laser scanning microscopy because it is excited at 490 nm and emits fluorescence around 510 nm. The fluorescence intensity of KMG-104 increases with increasing Mg2+ concentration and shows no response to Na, K, or Ca2+. The dissociation constant (Kd) for magnesium is 3 mM. KMG-104 can only track changes in Mg2+ under physiological conditions[2].
The novel magnesium ion indicator KMG-104 and the calcium ion indicator fura-2 were loaded into PC12 cells to investigate intracellular magnesium ions in the endoplasmic reticulum, mitochondria, and Mg-ATP[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 852057-94-4
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Appearance Solid
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Masse moléculaire 435.33
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Formule C23H11F2NO6
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Color Brown to red
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SMILES
O=C(C(C(O)=O)=C1)N2C(C=CC=C2)=C1C(C3=CC(F)=C(O)C=C3O4)=C5C4=CC(C(F)=C5)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvant et solubilité
DMSO : 50 mg/mL (114.86 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (280 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Kubota T, et al. Investigation of intracellular magnesium mobilization pathways I PC12 cells B simultaneous Mg-Ca fluorescent imaging. J Am Coll Nutr. 2004;23(6):742S-4S. [Content Brief]
[2]. Fujii T, et al. Design and synthesis of a FlAsH-type Mg2+ fluorescent probe for specific protein labeling. J Am Chem Soc. 2014;136(6):2374-2381. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2971 mL | 11.4855 mL | 22.9711 mL | 57.4277 mL |
| 5 mM | 0.4594 mL | 2.2971 mL | 4.5942 mL | 11.4855 mL | |
| 10 mM | 0.2297 mL | 1.1486 mL | 2.2971 mL | 5.7428 mL | |
| 15 mM | 0.1531 mL | 0.7657 mL | 1.5314 mL | 3.8285 mL | |
| 20 mM | 0.1149 mL | 0.5743 mL | 1.1486 mL | 2.8714 mL | |
| 25 mM | 0.0919 mL | 0.4594 mL | 0.9188 mL | 2.2971 mL | |
| 30 mM | 0.0766 mL | 0.3829 mL | 0.7657 mL | 1.9143 mL | |
| 40 mM | 0.0574 mL | 0.2871 mL | 0.5743 mL | 1.4357 mL | |
| 50 mM | 0.0459 mL | 0.2297 mL | 0.4594 mL | 1.1486 mL | |
| 60 mM | 0.0383 mL | 0.1914 mL | 0.3829 mL | 0.9571 mL | |
| 80 mM | 0.0287 mL | 0.1436 mL | 0.2871 mL | 0.7178 mL | |
| 100 mM | 0.0230 mL | 0.1149 mL | 0.2297 mL | 0.5743 mL |
KMG-104 Related Classifications
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