2. Membrane Transporter/Ion Channel Neuronal Signaling
  3. Calcium Channel
  4. Calcium ionophore I

Calcium ionophore I  (Synonyms: ETH 1001)

Cat. No.: HY-136460 Purity: ≥99.0%
COA Handling Instructions

Molarity Calculator

Calcium ionophore I (ETH 1001) is a selective Ca2+ ionophore for biological membranes. Calcium ionophore I can be used in Ca2+-selective microelectrodes that can be used for quantitative intracellular measurements of resting Ca2+-activities and of slowly changing Ca2+-levels.

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Calcium ionophore I Chemical Structure

Calcium ionophore I Chemical Structure

CAS No. : 58801-34-6

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Solvent
5 mg（14.60 mM * 500μL in Ethanol） USD 195 In-stock
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Based on 1 publication(s) in Google Scholar

Calcium ionophore I Related Antibodies

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Description

Calcium ionophore I (ETH 1001) is a selective Ca2+ ionophore for biological membranes. Calcium ionophore I can be used in Ca2+-selective microelectrodes that can be used for quantitative intracellular measurements of resting Ca2+-activities and of slowly changing Ca2+-levels[1][2].

Molecular Weight

684.99

Formula

C38H72N2O8
CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

O=C(OCC)CCCCCCCCCCCN(C)C(CO[C@H](C)[C@H](OCC(N(CCCCCCCCCCCC(OCC)=O)C)=O)C)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Solution, -20°C, 2 years
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (145.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (3.65 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (3.65 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥99.0%

COA (246 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4599 mL 7.2994 mL 14.5988 mL 36.4969 mL
5 mM 0.2920 mL 1.4599 mL 2.9198 mL 7.2994 mL
10 mM 0.1460 mL 0.7299 mL 1.4599 mL 3.6497 mL
15 mM 0.0973 mL 0.4866 mL 0.9733 mL 2.4331 mL
20 mM 0.0730 mL 0.3650 mL 0.7299 mL 1.8248 mL
25 mM 0.0584 mL 0.2920 mL 0.5840 mL 1.4599 mL
30 mM 0.0487 mL 0.2433 mL 0.4866 mL 1.2166 mL
40 mM 0.0365 mL 0.1825 mL 0.3650 mL 0.9124 mL
50 mM 0.0292 mL 0.1460 mL 0.2920 mL 0.7299 mL
60 mM 0.0243 mL 0.1217 mL 0.2433 mL 0.6083 mL
80 mM 0.0182 mL 0.0912 mL 0.1825 mL 0.4562 mL
100 mM 0.0146 mL 0.0730 mL 0.1460 mL 0.3650 mL
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Calcium ionophore I Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Calcium ionophore I58801-34-6ETH 1001ETH1001ETH-1001Calcium ChannelCa2+ channelsCa channelsCa2+ionophoremicroelectrodesInhibitorinhibitorinhibit

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