Carboxypyridostatin

Cat. No.: HY-112680 Purity: 99.41%: Data Sheet
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Carboxypyridostatin is a G-quadruplex ligand. Carboxypyridostatin has a highly molecular specificity to RNA on DNA G4s and reduces ATF-5 protein. Carboxypyridostatin reduces cell proliferation and hinders stress granule (SG) formation.

For research use only. We do not sell to patients.

CAS No. : 1417638-60-8

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5 mg		Ask For Quote & Lead Time
10 mg		Ask For Quote & Lead Time

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This product is a controlled substance and not for sale in your territory.

Other In-stock Forms of Carboxypyridostatin:

Carboxy pyridostatin trifluoroacetate salt

Other Forms of Carboxypyridostatin:

Carboxy pyridostatin trifluoroacetate salt In-stock

5 Publications Citing Use of MCE Carboxypyridostatin

Cell Proliferation/Viability Assay

Bio/Physico-chemical Assay

Microbiological Assay

: Carboxypyridostatin purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2022 May 6;50(8):4574-4600. [Abstract]; Cytotoxicity of the indicated G4-ligands (Carboxy pyridostatin trifluoroacetate salt (cPDS) etc.) for porcine kidney stable (PS) cells determined in a concentration range of 0–50 μM and expressed as a percentage of cell viability. PS cells were seeded in 96-well plates for 24 h, then treated with G4 ligands and incubated for 48 h.

: Carboxypyridostatin purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2022 May 6;50(8):4574-4600. [Abstract]; Fraction of full products of the polymerization reaction using four different templates, catalyzed by Japanese encephalitis virus RdRp, in the presence of three concentrations (50, 10 and 2 μM) of six tested G4 ligands (Carboxy pyridostatin trifluoroacetate salt (cPDS) etc.). Relative amount of full product in the presence of different concentrations of various G4 ligands normalized to the amount in control sample (no ligands).

: Carboxypyridostatin purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2022 May 6;50(8):4574-4600. [Abstract]; Inhibition of TBEV replication by the indicated G4-ligands. PS cell monolayers were treated with the indicated G4 ligands (50 μM) and simultaneously infected with TBEV strains Neudoerfl and Hypr at MOI of 0.1. The infected cells were then incubated with the compounds for 48 h. Following incubation, media supernatants were collected and viral titers were determined using a plaque assay and expressed as PFU/ml. Compounds PDS, Carboxy pyridostatin trifluoroacetate salt (cPDS), NMM, TMPyP4, berberine and PhenDC3 (marked in red) were selected for further evaluation of their antiviral potencies.

: Carboxypyridostatin purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2022 May 6;50(8):4574-4600. [Abstract]; Antiviral and cytotoxicity properties of G4-ligands (Carboxy pyridostatin trifluoroacetate salt (cPDS) etc.) incubated with virus-infected PS cells for 48 h.p.i.
^aDetermined from three independent experiments.
^bExpressed as a 50% reduction in viral titers and calculated as inflection points of sigmoidal inhibitory curves, which were obtained by a nonlinear fit of transformed inhibitor concentrations versus normalized response using GraphPad Prism 7.04.
^cExpressed as a 50% reduction in cell viability and calculated as inflection points of sigmoidal viability curves, which were obtained by a nonlinear fit of transformed inhibitor concentrations versus normalized response, see ^b.
^dWe were not able to evaluate the anti-TBEV effects of TMPyP4 accurately. Repeated virus cultivations in the presence of TMPyP4 provided extremely variable results that were not consistent enough to calculate the exact EC₅₀ values.
^eNo antiviral effect was observed at the lowest non-cytotoxic concentration of 5 μM.
^fAntiviral activities of TO and CV were not determined, as both compounds were highly cytotoxic (reduction of cell viability by ≥50%) in the tested concentration range (1.6-50 μM).
N.D.; not determined

: Carboxypyridostatin purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2022 May 6;50(8):4574-4600. [Abstract]; Fluorescence staining of PS cell culture infected with the TBEV Neudoerfl strain at a MOI of 0.1 in the presence of G4 ligands (Carboxy pyridostatin trifluoroacetate salt (cPDS) etc.) at the indicated concentrations. PS cells were fixed on slides at 48 h post-infection, stained with mouse flavivirus-specific antibody as a primary antibody, and anti-mouse goat secondary antibody conjugated with with FITC (green) and counterstained with DAPI (blue). Scale bars, 200 μm.

: Carboxypyridostatin purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2022 May 6;50(8):4574-4600. [Abstract]; Fluorescence staining of PS cell culture infected with the TBEV Hypr strain at a MOI of 0.1 in the presence of G4 ligands (Carboxy pyridostatin trifluoroacetate salt (cPDS) etc.) at the indicated concentrations. PS cells were fixed on slides at 48 h post-infection, stained with mouse flavivirus-specific antibody as a primary antibody, and anti-mouse goat secondary antibody conjugated with with FITC (green) and counterstained with DAPI (blue). Scale bars, 200 μm.

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Description

In Vitro

Carboxypyridostatin (1-25 μM; 3 days) reduces the size of adult SVZ-derived neurospheres and the number of cells in neurospheres, and blocks proliferation and neurosphere fusion in adult SVZ-derived neurosphere cultures^[1].
Carboxypyridostatin (10 μM; 24 h) hinders stress granule (SG) formation in U2OS cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Carboxypyridostatin (10 mg/kg; i.p.; every 12 h; 3 injections) significantly reduces the number of PCNA-expressing cells in the adult SVZ and increases the number of OLIG2-expressing cells in the corpus callosum in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 mice (both sexes, 1-2 months old)^[1]
Dosage:	10 mg/kg (dissolved in phosphate-buffered saline)
Administration:	Intraperitoneal injection, every 12 h, 3 injections
Result:	Reduced the number of PCNA-expressing cells in the adult SVZ of mice, indicating a decrease in cell proliferation. Increased the number of OLIG2-expressing cells in the corpus callosum. Showed no increase in OLIG2-expression in the SVZ. Affected the differentiation of neural stem and progenitor cells in different brain regions.

Molecular Weight

706.71

Formula

C₃₅H₃₄N₁₀O₇

CAS No.

1417638-60-8

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)CCN1N=NC(COC2=CC(C(NC3=NC4=CC=CC=C4C(OCCN)=C3)=O)=NC(C(NC5=NC6=CC=CC=C6C(OCCN)=C5)=O)=C2)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 1 mg/mL (1.42 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4150 mL	7.0750 mL	14.1501 mL
5 mM	---	---	---
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.41%

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Data Sheet (278 KB) SDS (251 KB)

COA (255 KB) HNMR (491 KB) LCMS (97 KB)

Handling Instructions (2659 KB)

References

[1]. Goldberg DC, et al. Manipulating Adult Neural Stem and Progenitor Cells with G-Quadruplex Ligands. ACS Chem Neurosci. 2020;11(10):1504-1518. [Content Brief]

[2]. Rocca R, et al. Molecular recognition of a carboxy pyridostatin toward G-quadruplex structures: Why does it prefer RNA? Chem Biol Drug Des. 2017 Nov;90(5):919-925. [Content Brief]

[3]. Turner M, et al. rG4detector, a novel RNA G-quadruplex predictor, uncovers their impact on stress granule formation. Nucleic Acids Res. 2022 Nov 11;50(20):11426-11441. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO

1 mM

1.4150 mL

7.0750 mL

14.1501 mL

35.3752 mL