Carboxypyridostatin

Carboxypyridostatin purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2022 May 6;50(8):4574-4600. [Abstract]

Customer Validation
Cytotoxicity of the indicated G4-ligands (Carboxy pyridostatin trifluoroacetate salt (cPDS) etc.) for porcine kidney stable (PS) cells determined in a concentration range of 0–50 μM and expressed as a percentage of cell viability. PS cells were seeded in 96-well plates for 24 h, then treated with G4 ligands and incubated for 48 h.

Carboxypyridostatin purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2022 May 6;50(8):4574-4600. [Abstract]

Customer Validation
Fraction of full products of the polymerization reaction using four different templates, catalyzed by Japanese encephalitis virus RdRp, in the presence of three concentrations (50, 10 and 2 μM) of six tested G4 ligands (Carboxy pyridostatin trifluoroacetate salt (cPDS) etc.). Relative amount of full product in the presence of different concentrations of various G4 ligands normalized to the amount in control sample (no ligands).

Carboxypyridostatin purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2022 May 6;50(8):4574-4600. [Abstract]

Customer Validation
Inhibition of TBEV replication by the indicated G4-ligands. PS cell monolayers were treated with the indicated G4 ligands (50 μM) and simultaneously infected with TBEV strains Neudoerfl and Hypr at MOI of 0.1. The infected cells were then incubated with the compounds for 48 h. Following incubation, media supernatants were collected and viral titers were determined using a plaque assay and expressed as PFU/ml. Compounds PDS, Carboxy pyridostatin trifluoroacetate salt (cPDS), NMM, TMPyP4, berberine and PhenDC3 (marked in red) were selected for further evaluation of their antiviral potencies.

Carboxypyridostatin purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2022 May 6;50(8):4574-4600. [Abstract]

Customer Validation
Antiviral and cytotoxicity properties of G4-ligands (Carboxy pyridostatin trifluoroacetate salt (cPDS) etc.) incubated with virus-infected PS cells for 48 h.p.i.
^aDetermined from three independent experiments.
^bExpressed as a 50% reduction in viral titers and calculated as inflection points of sigmoidal inhibitory curves, which were obtained by a nonlinear fit of transformed inhibitor concentrations versus normalized response using GraphPad Prism 7.04.
^cExpressed as a 50% reduction in cell viability and calculated as inflection points of sigmoidal viability curves, which were obtained by a nonlinear fit of transformed inhibitor concentrations versus normalized response, see ^b.
^dWe were not able to evaluate the anti-TBEV effects of TMPyP4 accurately. Repeated virus cultivations in the presence of TMPyP4 provided extremely variable results that were not consistent enough to calculate the exact EC₅₀ values.
^eNo antiviral effect was observed at the lowest non-cytotoxic concentration of 5 μM.
^fAntiviral activities of TO and CV were not determined, as both compounds were highly cytotoxic (reduction of cell viability by ≥50%) in the tested concentration range (1.6-50 μM).
N.D.; not determined

Carboxypyridostatin purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2022 May 6;50(8):4574-4600. [Abstract]

Customer Validation
Fluorescence staining of PS cell culture infected with the TBEV Neudoerfl strain at a MOI of 0.1 in the presence of G4 ligands (Carboxy pyridostatin trifluoroacetate salt (cPDS) etc.) at the indicated concentrations. PS cells were fixed on slides at 48 h post-infection, stained with mouse flavivirus-specific antibody as a primary antibody, and anti-mouse goat secondary antibody conjugated with with FITC (green) and counterstained with DAPI (blue). Scale bars, 200 μm.

Carboxypyridostatin purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2022 May 6;50(8):4574-4600. [Abstract]

Customer Validation
Fluorescence staining of PS cell culture infected with the TBEV Hypr strain at a MOI of 0.1 in the presence of G4 ligands (Carboxy pyridostatin trifluoroacetate salt (cPDS) etc.) at the indicated concentrations. PS cells were fixed on slides at 48 h post-infection, stained with mouse flavivirus-specific antibody as a primary antibody, and anti-mouse goat secondary antibody conjugated with with FITC (green) and counterstained with DAPI (blue). Scale bars, 200 μm.