168482-84-6
Chemical Structure
Calcein Blue AM
- CAS No.: 168482-84-6
- Formula:C21H23NO11
- Molecular Weight:465.41
IUPAC Name: bis(acetoxymethyl) 2,2'-(((7-hydroxy-4-methyl-2-oxo-2H-chromen-8-yl)methyl)azanediyl)diacetate
InChIKey: GSFJGCPBBHLSPH-UHFFFAOYSA-N
SMILES: OC1=CC=C2C(C)=CC(OC2=C1CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=O
Biological Activity: Calcein Blue AM is a cell staining agent. Calcein Blue AM serves as a substrate for intracellular esterases, which hydrolyze its acetoxymethyl (AM) ester group to convert the non-fluorescent reagent into fluorescent Calcein Blue. Calcein Blue AM passively penetrates the plasma membrane of both live and dead cells; it is converted into a polar fluorescent product and retained in the cytoplasm of live cells, whereas dead cells cannot retain the dye. Calcein Blue AM exhibits significant dye leakage, and its fluorescence intensity gradually decreases over time. Calcein Blue AM can be used for endpoint-based cell viability assays and short-term cell tracking experiments with a duration of no more than 4 hours.
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Calcein Blue AM | 99% | Calcein Blue AM is a cell staining agent. Calcein Blue AM serves as a substrate for intracellular esterases, which hydrolyze its acetoxymethyl (AM) ester group to convert the non-fluorescent reagent into fluorescent Calcein Blue. Calcein Blue AM passively penetrates the plasma membrane of both live and dead cells; it is converted into a polar fluorescent product and retained in the cytoplasm of live cells, whereas dead cells cannot retain the dye. Calcein Blue AM exhibits significant dye leakage, and its fluorescence intensity gradually decreases over time. Calcein Blue AM can be used for endpoint-based cell viability assays and short-term cell tracking experiments with a duration of no more than 4 hours. | ||||||||||||||||||||
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