Ceratamine A
Ceratamine A is an antimitotic heterocyclic alkaloid isolated from the marine sponge Pseudoceratina sp., acts as a microtubule-stabilizing agent. Ceratamine A exhibits cytotoxicity against human cancer cell lines.
For research use only. We do not sell to patients.
- CAS No.: 634151-15-8
- Formula: C17H16Br2N4O2
- Molecular Weight:468.14
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Description
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| A2780 | IC50 |
11.2 μM
Compound: Ceratamine A
|
Cytotoxicity against human A2780 cells assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against human A2780 cells assessed as growth inhibition after 72 hrs by MTT assay
|
[PMID: 29433924] |
| A549 | IC50 |
26.8 μM
Compound: Ceratamine A
|
Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by MTT assay
|
[PMID: 29433924] |
| BGC-823 | IC50 |
20 μM
Compound: Ceratamine A
|
Cytotoxicity against human BGC823 cells assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against human BGC823 cells assessed as growth inhibition after 72 hrs by MTT assay
|
[PMID: 29433924] |
| HCT-116 | IC50 |
12.4 μM
Compound: Ceratamine A
|
Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay
|
[PMID: 29433924] |
| HepG2 | IC50 |
7.8 μM
Compound: Ceratamine A
|
Cytotoxicity against human HepG2 cells assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against human HepG2 cells assessed as growth inhibition after 72 hrs by MTT assay
|
[PMID: 29433924] |
| MCF7 | IC50 |
5 μg/mL
Compound: 1
|
Antimitotic activity in human MCF7mp53 cells assessed as induction of mitotic arrest after 20 hrs
Antimitotic activity in human MCF7mp53 cells assessed as induction of mitotic arrest after 20 hrs
|
[PMID: 20945907] |
Chemical Information
-
CAS No. 634151-15-8
-
Molecular Weight 468.14
-
Formula C17H16Br2N4O2
-
SMILES
O=C(N(C)C=C1)C(CC2=CC(Br)=C(OC)C(Br)=C2)=C3C1=NC(NC)=N3
-
Structure Classification
-
Initial Source
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
-
Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
-
Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
-
Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
[1]. Manzo E, et al. Ceratamines A and B, antimitotic heterocyclic alkaloids isolated from the marine sponge Pseudoceratina sp. collected in Papua New Guinea. Org Lett. 2003 Nov 27;5(24):4591-4. [Content Brief]
[2]. Nodwell M, et al. Synthetic approaches to the microtubule-stabilizing sponge alkaloid ceratamine A and desbromo analogues. J Org Chem. 2009 Feb 6;74(3):995-1006. [Content Brief]
[3]. Pan X, et al. Synthesis and cytotoxicity of novel imidazo[4,5-d]azepine compounds derived from marine natural product ceratamine A. Bioorg Med Chem Lett. 2018 Mar 1;28(5):866-868. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Ceratamine A Related Classifications
HY-N6997 Related Classifications
Powered by Bioz