16-Azidohexadecanoic acid
Based on 1 Customer Validation
16-Azidohexadecanoic acid is a fatty acid metabolism probe and a substrate of fatty acid transport protein (FATP). 16-Azidohexadecanoic acid serves as a metabolic probe for fatty acid analysis via click chemistry and HPLC/MS detection.
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- Reinheit : 98.0%
- CAS. Nr.: 112668-54-9
- Formel: C16H31N3O2
- Molecular Weight:297.44
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Speicherung:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Beschreibung
In Vitro
16-Azidohexadecanoic acid (1 mM; 24 h) is degraded over time in E. coli ilvE and fadA mutants, but not in fadE mutants, indicating that FadE is required for its β-oxidation[1].
16-Azidohexadecanoic acid (1 mM; 24 h) serves as a substrate for the desaturase of P. urativorans, confirming that this enzyme is involved in fatty acid modification[1].
16-Azidohexadecanoic acid is used to synthesize the azide-modified nucleotide dC^C16N3TP via a HATU-promoted coupling reaction in 35% yield[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS. Nr. 112668-54-9
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Appearance Solid
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Molecular Weight 297.44
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Formel C16H31N3O2
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Color White to off-white
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SMILES
O=C(O)CCCCCCCCCCCCCCCN=[N+]=[N-]
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (336.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.41 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.41 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protokoll
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Reinheit & Dokumentation
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Data Sheet (266 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Pérez AJ, et al. ω-Azido fatty acids as probes to detect fatty acid biosynthesis, degradation, and modification. Journal of lipid research. 2014 Sep;55(9):1897-901. [Content Brief]
[3]. Balintová J, et al. Antibody-nucleotide conjugate as a substrate for DNA polymerases. Chemical science. 2018 Sep 21;9(35):7122-7125. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3620 mL | 16.8101 mL | 33.6202 mL | 84.0506 mL |
| 5 mM | 0.6724 mL | 3.3620 mL | 6.7240 mL | 16.8101 mL | |
| 10 mM | 0.3362 mL | 1.6810 mL | 3.3620 mL | 8.4051 mL | |
| 15 mM | 0.2241 mL | 1.1207 mL | 2.2413 mL | 5.6034 mL | |
| 20 mM | 0.1681 mL | 0.8405 mL | 1.6810 mL | 4.2025 mL | |
| 25 mM | 0.1345 mL | 0.6724 mL | 1.3448 mL | 3.3620 mL | |
| 30 mM | 0.1121 mL | 0.5603 mL | 1.1207 mL | 2.8017 mL | |
| 40 mM | 0.0841 mL | 0.4203 mL | 0.8405 mL | 2.1013 mL | |
| 50 mM | 0.0672 mL | 0.3362 mL | 0.6724 mL | 1.6810 mL | |
| 60 mM | 0.0560 mL | 0.2802 mL | 0.5603 mL | 1.4008 mL | |
| 80 mM | 0.0420 mL | 0.2101 mL | 0.4203 mL | 1.0506 mL | |
| 100 mM | 0.0336 mL | 0.1681 mL | 0.3362 mL | 0.8405 mL |
16-Azidohexadecanoic acid Related Classifications
HY-147309 Related Classifications
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