Bz-VGR-AMC
Bz-VGR-AMC is a fluorescent substrate for the trypsin-like activity of the 20S proteasome. Bz-VGR-AMC can be used to measure the trypsin-like (β2) activity of the 20S proteasome (Ex/Em = 360/460 nm).
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- CAS. Nr.: 87779-49-5
- Formel: C30H37N7O6
- Molecular Weight:591.66
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Take the tissue sample to be tested, dilute it with ddH2O, and mix it with the reaction buffer (250 mM HEPES, pH 7.5, 5 mM EDTA, 0.5% NP-40, 0.01% SDS, 5 mM ATP).
2. Add the fluorescent peptide Bz-VGR-AMC (10 μM) to the sample.
3. Incubate the reaction at 37°C for 30 minutes.
4. Monitor the fluorescence on a fluorescence microplate reader at a wavelength of Ex/Em = 360/460 nm.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 87779-49-5
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Molecular Weight 591.66
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Formel C30H37N7O6
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Sequence
{Bz}-Val-Gly-Arg-{AMC}
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Sequence Shortening
{Bz}-VGR-{AMC}
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Reinheit & Dokumentation
Verweise
[1]. Zhong YL, et al. Celastrol induce apoptosis of human multiple myeloma cells involving inhibition of proteasome activity. Eur J Pharmacol. 2019 Jun 15;853:184-192. [Content Brief]
[2]. Jarome TJ, et al. CaMKII, but not protein kinase A, regulates Rpt6 phosphorylation and proteasome activity during the formation of long-term memories. Front Behav Neurosci. 2013 Aug 30;7:115. [Content Brief]
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Bz-VGR-AMC Related Classifications
HY-137897 Related Classifications
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