Bz-VGR-AMC TFA
Based on 1 Customer Validation
Bz-VGR-AMC TFA is a fluorescent substrate for the trypsin-like activity of the 20S proteasome. Bz-VGR-AMCTFA can be used to measure the trypsin-like (β2) activity of the 20S proteasome (Ex/Em = 360/460 nm).
For research use only. We do not sell to patients.
- Formula: C30H37N7O6·xC2HF3O2
- Molecular Weight:591.66 (free base)
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Take the tissue sample to be tested, dilute it with ddH2O, and mix it with the reaction buffer (250 mM HEPES, pH 7.5, 5 mM EDTA, 0.5% NP-40, 0.01% SDS, 5 mM ATP).
2. Add the fluorescent peptide Bz-VGR-AMC (10 μM) TFA to the sample.
3. Incubate the reaction at 37°C for 30 minutes.
4. Monitor the fluorescence on a fluorescence microplate reader at a wavelength of Ex/Em = 360/460 nm.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 591.66 (free base)
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Formula C30H37N7O6·xC2HF3O2
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SMILES
CC(C(C(O1)=C2)=CC=C2NC([C@H](CCCNC(N)=N)NC(CNC([C@H](C(C)C)NC(C3=CC=CC=C3)=O)=O)=O)=O)=CC1=O.O=C(O)C(F)(F)F.[x]
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Sequence
Bz-Val-Gly-Arg-AMC
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Sequence Shortening
Bz-VGR-AMC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Purity & Documentation
References
[1]. Zhong YL, et al. Celastrol induce apoptosis of human multiple myeloma cells involving inhibition of proteasome activity. Eur J Pharmacol. 2019 Jun 15;853:184-192. [Content Brief]
[2]. Jarome TJ, et al. CaMKII, but not protein kinase A, regulates Rpt6 phosphorylation and proteasome activity during the formation of long-term memories. Front Behav Neurosci. 2013 Aug 30;7:115. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Bz-VGR-AMC TFA Related Classifications
HY-137897A Related Classifications
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