EGFR-IN-71
EGFR-IN-71 is a potent narrow spectrum epidermal growth factor receptor (EGFR) inhibitor with IC50 values of 3.7 μM. EGFR-IN-71 can be used for researching chordoma. EGFR-IN-71 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
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- CAS. Nr.: 2676155-98-7
- Formel: C16H9ClIN3
- Molecular Weight:405.62
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
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Chemical Information
- Protokoll
- Reinheit & Dokumentation
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Help & FAQs
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Biologische Aktivität
Beschreibung
IC50 & Target
IC50: 3.7 μM (EGFR)[1]
In Vitro
EGFR-IN-71 (compound 41) (0-100 μM; 72 h) has inhibitory activity against U-CH1, U-CH2, CH22, UM-Chor1, U-CH12 and U-CH7 chordoma cell lines[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:U-CH1, U-CH2, CH22, UM-Chor1, U-CH12 and U-CH7
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Concentration:0-100 μM
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Incubation Time:72 h
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Result:Exhibited inhibitory activity against U-CH1, U-CH2, CH22, UM-Chor1, U-CH12 and U-CH7 chordoma cell lines with IC50s of 9.1 μM, 16 μM, 0.48 μM, 25 μM, 0.96 μM and 8.0 μM, respectively.
Chemical Information
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CAS. Nr. 2676155-98-7
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Molecular Weight 405.62
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Formel C16H9ClIN3
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SMILES
IC1=CC2=NC=NC(NC3=CC=C(Cl)C(C#C)=C3)=C2C=C1
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Protokoll
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
EGFR-IN-71 Related Classifications
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