CellTracker Blue CMAC
Based on 7 publication(s) in Google Scholar
CellTracker Blue CMAC is a non-fluorescent cell membrane permeable dye. The chloromethyl groups of CellTracker Blue CMAC are enzymatically cleaved by intracellular glutathione (GSH) to generate a fluorescent product (blue fluorescence, Ex/Em: 360/460 nm). CellTracker Blue CMAC is suitable for long-term cell tracking (up to 72 hours) and cell proliferation studies, and can also quantify GSH levels.
For research use only. We do not sell to patients.
- Purity: 98.84%
- CAS No.: 147963-22-2
- Formula: C10H8ClNO2
- Molecular Weight:209.63
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) CellTracker Blue CMAC
More
Customer Validation & Images
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Imaging/Staining
Biological Activity
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Dye preparation
Stock solution: Dissolve in DMSO to a final concentration of 10 mM (protect from light and store in aliquots).
Working solution: Dilute the stock solution to a working concentration of 0.5-25 μM in serum-free medium. Preheat the staining working solution to 37 °C.
2. Cell labeling
Remove the cell culture medium and wash the cells with PBS.
Add the dye working solution and incubate at 37 °C for 30 minutes.
Remove the dye and wash 3 times with pre-warmed culture medium.
Change to fresh culture medium and continue culturing or experiment (fluorescence signal reaches peak value within 1-2 hours).
3. Detection
Fluorescence microscopy: (Ex 360 nm, Em 460 nm).
4. Precautions
4.1 Please adjust the CellTracker Blue CMAC working solution concentration, incubation temperature and time according to actual conditions.
4.2 This product is limited to scientific research by professionals and shall not be used for clinical diagnosis or treatment, nor for food or medicine.
4.3 For your safety and health, please wear a lab coat and disposable gloves when operating.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 147963-22-2
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Appearance Solid
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Molecular Weight 209.63
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Formula C10H8ClNO2
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Color White to light yellow
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SMILES
O=C1OC2=C(C(CCl)=C1)C=CC(N)=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (7)
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Journal Impact Factor
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Most Recent
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Nat Commun
Impairing the interaction between Erg11 and cytochrome P450 reductase Ncp1 enhances azoles' antifungal activities. [Abstract]2025 Jul 24;16(1):6821. PMID: 40707518
CellTracker Blue CMAC purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 24;16(1):6821. [Abstract]
Representativeimages depicting the co-localization the green region (indicative of Ca2+ by Fluo-3AM) in relation to the blue region (marked by Cell Tracker Blue（25 μM; 30 min） as vacuoles) wereobtained from ERG11/erg11Δ and erg11m/erg11Δ mutants.
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Nat Commun
A potent protective bispecific nanobody targeting Herpes simplex virus gD reveals vulnerable epitope for neutralizing. [Abstract]2025 May 6;16(1):4196. PMID: 40328740 -
Adv Sci (Weinh)
Photo-Empowered Macrophage-Based Drug Delivery System Overcomes Motility Suppression and Significantly Enhances Deep Tumor Drug Delivery. [Abstract]2025 Nov 9:e15349. PMID: 41208150 -
Adv Sci (Weinh)
Retromer Regulates Macro- and Micro-Autophagy via Distinct Vacuolar Proteases in the Rice Blast Fungus. [Abstract]2025 Aug 21:e10068. PMID: 40842061
CellTracker Blue CMAC purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 21:e10068. [Abstract]
Mycelia expressing MoPrb1-GFP were stained with CellTracker Blue CMAC (10 µM) and observed using a confocal microscope.
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Curr Microbiol
2025 Dec 27;83(2):100. PMID: 41460255 -
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CellTracker Blue CMAC purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 Sep 17.
Colocalization between Erg11-GFP and vacuole in cells precultured at 30 °C or 37 °C for 1 h. Pseudo-color was used to display the blue fluorescence of the vacuole dye (CellTracker Blue CMAC; 100 μM; 30 min) as red. The white arrow indicated vacuoles with Erg11-GFP.
Solvent & Solubility
DMSO : 125 mg/mL (596.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[2]. King N, et al. A new method of quantifying glutathione levels in freshly isolated single superfused rat cardiomyocytes. J Pharmacol Toxicol Methods. 2004 Nov-Dec;50(3):215-22. [Content Brief]
[3]. Dludla PV, et al. A dose-dependent effect of dimethyl sulfoxide on lipid content, cell viability and oxidative stress in 3T3-L1 adipocytes. Toxicol Rep. 2018 Oct 4;5:1014-1020. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.7703 mL | 23.8515 mL | 47.7031 mL | 119.2577 mL |
| 5 mM | 0.9541 mL | 4.7703 mL | 9.5406 mL | 23.8515 mL | |
| 10 mM | 0.4770 mL | 2.3852 mL | 4.7703 mL | 11.9258 mL | |
| 15 mM | 0.3180 mL | 1.5901 mL | 3.1802 mL | 7.9505 mL | |
| 20 mM | 0.2385 mL | 1.1926 mL | 2.3852 mL | 5.9629 mL | |
| 25 mM | 0.1908 mL | 0.9541 mL | 1.9081 mL | 4.7703 mL | |
| 30 mM | 0.1590 mL | 0.7951 mL | 1.5901 mL | 3.9753 mL | |
| 40 mM | 0.1193 mL | 0.5963 mL | 1.1926 mL | 2.9814 mL | |
| 50 mM | 0.0954 mL | 0.4770 mL | 0.9541 mL | 2.3852 mL | |
| 60 mM | 0.0795 mL | 0.3975 mL | 0.7951 mL | 1.9876 mL | |
| 80 mM | 0.0596 mL | 0.2981 mL | 0.5963 mL | 1.4907 mL | |
| 100 mM | 0.0477 mL | 0.2385 mL | 0.4770 mL | 1.1926 mL |
CellTracker Blue CMAC Related Classifications
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