Safranin
Based on 1 Customer Validation
Safranin (Safranin T) is an important and classical phenazinium dye. Safranin has been extensively used in the academic field as a spectroscopic probe and indicator. Safranin possesses a planar structure and cationic charge. Safranin can readily intercalate into biological macromolecules, including DNA and proteins. Safranin has antibacterial effects against gram-positive bacteria (S. aureus). Safranin can be used as a redox indicator in the determination of metal ion concentration.
For research use only. We do not sell to patients.
- CAS No.: 477-73-6
- Formula: C20H19ClN4
- Molecular Weight:350.85
-
Storage:
RT, sealed storage, away from moisture and light.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Safranin (0.1% (w/v), 1 min) differentially stains the cell cycle of plant cells[2].
Safranin (up to 2 μM) inhibits Complex I-driven OXPHOS (in mouse brain homogenates) capacity[3].
Safranin (0.025%) shows antibacterial activity against gram-positive bacteria (S. aureus)[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 477-73-6
-
Appearance Solid
-
Molecular Weight 350.85
-
Formula C20H19ClN4
-
Color Purple to black
-
SMILES
CC1=CC2=NC3=C([N+](C4=CC=CC=C4)=C2C=C1N)C=C(N)C(C)=C3.[Cl-]
-
Synonyms
Safranine T
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
RT, sealed storage, away from moisture and light
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
DMSO : 25 mg/mL (71.26 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (274 KB)
-
SDS (476 KB)
- English - EN (476 KB)
- Français - FR (476 KB)
- Deutsch - DE (476 KB)
- Norwegian - NO (476 KB)
- Español - ES (476 KB)
- Swedish - SV (476 KB)
- Italian - IT (476 KB)
- Korean - KR (476 KB)
- Portuguese - PT (476 KB)
-
Handling Instructions (2659 KB)
References
[1]. Wan H, et al. Structure characterization and optical properties investigation of the four main components of the classical phenazinium dye Safranin O. Analyst. 2019;144(24):7149-7156. [Content Brief]
[2]. Swain D, et al. Differential staining of the cell cycle of plant cells using safranin and indigo-picrocarmine. Stain Technol. 1990;65(4):197-204. [Content Brief]
[3]. Krumschnabel G, et al. Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol. 2014;542:163-81. [Content Brief]
[4]. Al-Khikani F, et al. The Antibacterial Action of Safranin and Gentian Violet. Rambam Maimonides Med J. 2022 Jul 31;13(3):e0018. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8502 mL | 14.2511 mL | 28.5022 mL | 71.2555 mL |
| 5 mM | 0.5700 mL | 2.8502 mL | 5.7004 mL | 14.2511 mL | |
| 10 mM | 0.2850 mL | 1.4251 mL | 2.8502 mL | 7.1256 mL | |
| 15 mM | 0.1900 mL | 0.9501 mL | 1.9001 mL | 4.7504 mL | |
| 20 mM | 0.1425 mL | 0.7126 mL | 1.4251 mL | 3.5628 mL | |
| 25 mM | 0.1140 mL | 0.5700 mL | 1.1401 mL | 2.8502 mL | |
| 30 mM | 0.0950 mL | 0.4750 mL | 0.9501 mL | 2.3752 mL | |
| 40 mM | 0.0713 mL | 0.3563 mL | 0.7126 mL | 1.7814 mL | |
| 50 mM | 0.0570 mL | 0.2850 mL | 0.5700 mL | 1.4251 mL | |
| 60 mM | 0.0475 mL | 0.2375 mL | 0.4750 mL | 1.1876 mL |
Safranin Related Classifications
HY-D0215 Related Classifications
Powered by Bioz