DKFZ-633
DKFZ-633 is a potent KLK6 inhibitor and KLK6-specific probe with an IC50 of 250 nM against human KLK6. DKFZ-633 covalently labels active KLK6 in a specific manner, and successfully pulls down and captures endogenous active KLK6 from cell-conditioned media. DKFZ-633 serves as a critical tool for investigating the function of KLK6 and its regulatory mechanisms in head and neck cancer.
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- CAS No.: 2244425-14-5
- Formule: C26H24N4O3
- Masse moléculaire:440.50
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
DKFZ-633 (compound 44) (0.25 μM; 30 min) inhibits recombinant triple-mutant human KLK6, with an IC50 of 0.25 μM after 30 min of pre-incubation at room temperature[1].
DKFZ-633 (12-25 μM; 1 h; room temperature) pulls down active endogenous human KLK6 from FaDu cell-conditioned medium in a dose-dependent manner[1].
DKFZ-633 (50 μM; 1 h; room temperature) pulls down active endogenous human KLK6 from the conditioned media of various human cancer cell lines, including two cell-dependent glycoforms[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:FaDu head and neck squamous cell carcinoma cells
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Concentration:100, 300, 500 nM
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Incubation Time:48 h (daily treatment)
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Result:Increased BrdU-positive FaDu cells in a dose-dependent manner: ~45% (100 nM), ~50% (300 nM), ~60% (500 nM), compared to ~45% in vehicle control.
Induced a dose-dependent phenotypic change in FaDu cells, mimicking the effect of KLK6 shRNA silencing.
Chemical Information
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CAS No. 2244425-14-5
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Masse moléculaire 440.50
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Formule C26H24N4O3
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SMILES
O=C(OCC(=O)NC=1C(=NN(C=2C=CC=CC2)C1C)C)CC=3C=4C=C(C#C)C=CC4NC3C
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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