Poly(A) polymerase
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Poly(A) polymerase catalyzes the incorporation of ATP into the RNA 3' end in the form of AMP, that is, adding a polyadenosine tail at the RNA 3' end. Poly(A) polymerase increases RNA stability and increases mRNA translation efficiency in eukaryotic cells. Poly(A) polymerase has high tailing efficiency and can add 20-200 A bases to the 3' end of RNA.
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- CAS No.: 9026-30-6
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Description
In Vitro
| Specification | Type | Components | Volume | Quantity |
| 100 U | A | Poly(A) Polymerase (5U/μL) | 100 U | 1 |
| 100 U | B | 10 x Poly(A) Polymerase Buffer | 1 mL | 1 |
| 100 U | C | ATP (10 mM) | 0.1 mL | 1 |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 9026-30-6
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[Poly(A) polymerase]
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
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Fiche technique (273 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Li W, et al. The novel poly(A) polymerase Star-PAP is a signal-regulated switch at the 3'-end of mRNAs. Adv Biol Regul. 2013 Jan;53(1):64-76. [Content Brief]
[2]. Chen LS, et al. Chain termination and inhibition of mammalian poly(A) polymerase by modified ATP analogues. Biochem Pharmacol. 2010 Mar 1;79(5):669-77. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Poly(A) polymerase Related Classifications
HY-E70099 Related Classifications
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