RNase H2
Based on 1 Customer Validation
RNase H2 is the predominant source of RNase H activity in mammalian and human cells. RNase H2 protects genome integrity. RNase H2 has been associated with ribonucleotide removal from genomic DNA in yeast and mouse, where it is required for embryonic development.
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Activité biologique
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Chemical Information
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
Product Information
This product is recombinantly expressed in E. coli. In addition to degrading the RNA chain in the R-loop and DNA-RNA complex chain, it can also specifically cut single ribonucleotides in the DNA double-stranded chain to repair the DNA double-stranded chain.
Storage buffer: 20 mM Tris-HCl, 100 mM KCI, 1 mM DTT, 1 mM EDTA, 50% Glycerol, pH 8.0
Instructions
1. Add 100 pmol double-stranded DNA substrate, 0.2-0.5 μL RNase H2, 5 μL reaction buffer (200 mM Tris-HCl, 100 mM (NH4) 2SO4, 100 mM KCl, 20 mM MgSO4, 1% Triton X-100, pH 8.8) according to the reaction system, and add Nuclease-free Water to make up to 50 μL;
2. After mixing, incubate the sample at 37°C for 30 min.
| Specification | Type | Components | Volume | Quantity |
| 100 U | A | RNase H2 | 100 U | 1 |
| 100 U | B | 10x RNase H2 Reaction Buffer | 200 μL | 2 |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
EC Number
3.1.26.4
Enzyme Activity
>5 U/μl
Unit Definition
One unit is defined as the amount of enzyme that can cleave 1 nmol of synthetic DNA/RNA hybrid double-stranded body substrate containing a single R-loop per min at 37°C
Chemical Information
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[RNase H2]
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
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Fiche technique (268 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Feng S, Cao Z. Is the role of human RNase H2 restricted to its enzyme activity? Prog Biophys Mol Biol. 2016 May;121(1):66-73. [Content Brief]
[2]. Chon H, et al. RNase H2 roles in genome integrity revealed by unlinking its activities. Nucleic Acids Res. 2013 Mar 1;41(5):3130-43. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
RNase H2 Related Classifications
HY-E70098 Related Classifications
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