2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease Anti-infection
  3. Reactive Oxygen Species Influenza Virus
  4. Cobalt protoporphyrin IX

Cobalt protoporphyrin IX  (Synonyms: Co-PPIX)

Cat. No.: HY-134608
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Cobalt protoporphyrin IX (Co-PPIX) is a potent and specific heme oxygenase-1 (HO-1) inducer. Cobalt protoporphyrin IX exhibits broad-spectrum antiviral activities against Influenza A virus (IAV).

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Cobalt protoporphyrin IX Chemical Structure

Cobalt protoporphyrin IX Chemical Structure

CAS No. : 14325-03-2

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Cobalt protoporphyrin IX Related Antibodies

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Description

Cobalt protoporphyrin IX (Co-PPIX) is a potent and specific heme oxygenase-1 (HO-1) inducer. Cobalt protoporphyrin IX exhibits broad-spectrum antiviral activities against Influenza A virus (IAV)[1].

In Vitro

Cobalt protoporphyrin IX (2 μM; 0-24 h) increases the intracellular protein levels of HO-1 in a time-dependent manner in MDCK cells and RAW264.7 cells[1].
Cobalt protoporphyrin IX shows antiviral activity against influenza viruses with IC50s of 0.40 ± 0.16, 0.42 ± 0.15, 0.46 ± 0.19, 0.34 ± 0.05 and 0.64 ± 0.30 μM against Empty Cell A/FortMonmouth /1/1947, A/TianjinJinnan /15/2009, A/Wuhan /359/1995, A/FujianTongan /196/2009 and BY/FujianXinluo /54/2006, respectively[1].
Cobalt protoporphyrin IX (0.25-2 μM; 18 or 24 h) inhibits IAV replication through augmenting IFN response, and the anti-IAV activity is not dependent on the catalytic function of HO-1[1].
Anti-IAV activity of HO-1 induced by Cobalt protoporphyrin IX (2 μM; 0-6 h) may depend on IRF3 phosphorylation and translocation, in order to enhance antiviral IFN response[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cobalt protoporphyrin IX Related Antibodies

Western Blot Analysis[1]

Cell Line: MDCK cells and RAW264.7 cells
Concentration: 2 μM
Incubation Time: 0, 3, 6, 12 and 24 h
Result: Significantly increased the intracellular protein levels of HO-1 in a time-dependent manner in MDCK cells and RAW264.7 cells.

Real Time qPCR[1]

Cell Line: RAW264.7 cells were infected with IAV A/Fort Monmouth/1/1947 (0.2 MOI)
Concentration: 0.25, 0.5, 1 and 2 μM
Incubation Time: 18 h
Result: Enhanced mRNA expressions of IFN-α/β, as well as protein expressions of some ISGs, such as IFN-inducible transmembrane protein 3 (IFITM3), double-stranded RNA-dependent protein kinase (PKR) and 2′-5′-oligoadenylate synthetase 1 (OAS1), in a dose-dependent manner.

Western Blot Analysis[1]

Cell Line: RAW264.7 cells or RAW264.7 cells infected with IAV A/Fort Monmouth/1/1947 (0.2 MOI)
Concentration: 2 μM
Incubation Time: 0, 2, 4 and 6 h
Result: Increased protein levels of IRF3 and p-IRF3. Promoted the cytoplasmic protein levels of IRF3 and p-IRF3 in RAW264.7 cells following the enhancement of HO-1 protein expression after 6 h. Induced nuclear IRF3 and nuclear p-IRF3 accumulation within 6 h of treatment.
Molecular Weight

619.58

Formula

C34H32CoN4O4
CAS No.
SMILES

CC1=C(CCC([O-])=O)C2=CC(C(CCC([O-])=O)=C3C)=[N]4C3=CC5=C(C)C(C=C)=C6C=C(C(C)=C7C=C)[N]8=C7C=C1[N-]2[Co+2]84[N-]56.[H+].[H+]
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Cobalt protoporphyrin IX Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cobalt protoporphyrin IX14325-03-2Co-PPIXReactive Oxygen SpeciesInfluenza VirusMDCKRAW264.7Empty Cell A/FortMonmouth /1/1947A/TianjinJinnan /15/2009A/Wuhan /359/1995A/FujianTongan /196/2009BY/FujianXinluo /54/2006Inhibitorinhibitorinhibit

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