Cobalt protoporphyrin IX
Cobalt protoporphyrin IX (Co-PPIX) is a potent and specific heme oxygenase-1 (HO-1) inducer. Cobalt protoporphyrin IX exhibits broad-spectrum antiviral activities against Influenza A virus (IAV).
For research use only. We do not sell to patients.
- CAS No.: 14325-03-2
- Formula: C34H32CoN4O4
- Molecular Weight:619.58
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Cobalt protoporphyrin IX (2 μM; 0-24 h) increases the intracellular protein levels of HO-1 in a time-dependent manner in MDCK cells and RAW264.7 cells[1].
Cobalt protoporphyrin IX shows antiviral activity against influenza viruses with IC50s of 0.40 ± 0.16, 0.42 ± 0.15, 0.46 ± 0.19, 0.34 ± 0.05 and 0.64 ± 0.30 μM against Empty Cell A/FortMonmouth /1/1947, A/TianjinJinnan /15/2009, A/Wuhan /359/1995, A/FujianTongan /196/2009 and BY/FujianXinluo /54/2006, respectively[1].
Cobalt protoporphyrin IX (0.25-2 μM; 18 or 24 h) inhibits IAV replication through augmenting IFN response, and the anti-IAV activity is not dependent on the catalytic function of HO-1[1].
Anti-IAV activity of HO-1 induced by Cobalt protoporphyrin IX (2 μM; 0-6 h) may depend on IRF3 phosphorylation and translocation, in order to enhance antiviral IFN response[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:MDCK cells and RAW264.7 cells
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Concentration:2 μM
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Incubation Time:0, 3, 6, 12 and 24 h
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Result:Significantly increased the intracellular protein levels of HO-1 in a time-dependent manner in MDCK cells and RAW264.7 cells.
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Cell Line:RAW264.7 cells were infected with IAV A/Fort Monmouth/1/1947 (0.2 MOI)
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Concentration:0.25, 0.5, 1 and 2 μM
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Incubation Time:18 h
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Result:Enhanced mRNA expressions of IFN-α/β, as well as protein expressions of some ISGs, such as IFN-inducible transmembrane protein 3 (IFITM3), double-stranded RNA-dependent protein kinase (PKR) and 2′-5′-oligoadenylate synthetase 1 (OAS1), in a dose-dependent manner.
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Cell Line:RAW264.7 cells or RAW264.7 cells infected with IAV A/Fort Monmouth/1/1947 (0.2 MOI)
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Concentration:2 μM
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Incubation Time:0, 2, 4 and 6 h
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Result:Increased protein levels of IRF3 and p-IRF3. Promoted the cytoplasmic protein levels of IRF3 and p-IRF3 in RAW264.7 cells following the enhancement of HO-1 protein expression after 6 h. Induced nuclear IRF3 and nuclear p-IRF3 accumulation within 6 h of treatment.
Chemical Information
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CAS No. 14325-03-2
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Appearance Solid
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Molecular Weight 619.58
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Formula C34H32CoN4O4
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Color Brown to black
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SMILES
CC1=C(CCC([O-])=O)C2=CC(C(CCC([O-])=O)=C3C)=[N]4C3=CC5=C(C)C(C=C)=C6C=C(C(C)=C7C=C)[N]8=C7C=C1[N-]2[Co+2]84[N-]56.[H+].[H+]
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Synonyms
Co-PPIX
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Protocols
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
Purity & Documentation
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Data Sheet (282 KB)
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SDS (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Cobalt protoporphyrin IX Related Classifications
HY-134608 Related Classifications
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