Crocin-4
Crocin-4, a carotenoid constituent of saffron, is a potent and brain-penetrant antioxidant agent. Crocin-4 can inhibit the aggregation and the concomitant deposition of Aβ fibrils in the brain. Crocin-4 can be used for the research of Alzheimer's Disease. Crocin-4 also exhibits antitumor and anti-inflammatory activities.
For research use only. We do not sell to patients.
- CAS No.: 55750-86-2
- Formula: C27H36O9
- Molecular Weight:504.57
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Crocin-4 (0.1-1000 μM; 24-72 h) do not compromise cell viability of neuron-like cells[1].
Crocin-4 (1 mM; 72 h) reduces total PSEN1, PSEN1 and PSEN2 complexes, BACE1, APP-C99 and sAPPα, while it increases PSEN1-CTF and PSEN2 in SH-SY5Y-APP cells[1].
Crocin-4 (1 mM; 72 h) suppresses GSK3β and ERK1/2 kinases in PC12-htau cells, and significantly reduces the levels and phosphorylation of tau on the pThr231 and pSer199/Ser202 epitopes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:Differentiated SH-SY5Y-APP cells
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Concentration:1 mM
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Incubation Time:72 hours
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Result:Significantly reduced total PSEN1 by 19%, and the PSEN1 and PSEN2 complexes by 81 and 65%, while it increased PSEN1-CTF (26%) and PSEN2 (43%).
The key molecules of the amyloidogenic pathway, BACE1 and APP-C99 were significantly reduced by 20 and 28%, respectively.
The non-amyloidogenic pathway product sAPPα was also reduced, by 44%, while APP-C83 remained unaltered.
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Cell Line:Differentiated PC12-htau cells
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Concentration:1 mM
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Incubation Time:72 hours
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Result:Significantly reduced total tau levels (by 32%) and tau phosphorylation (pThr231 and pSer199/Ser202-tau by 22 and 75%, respectively).
Downregulates the active and inactive forms of GSK3β (total GSK3β by 34%, pSer9-GSK3β by 30%) and ERK1/2 (total ERK2 by 37%, pERK1 by 40%, pERK2 by 50%).
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 55750-86-2
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Molecular Weight 504.57
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Formula C27H36O9
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SMILES
COC(/C(C)=C/C=C/C(C)=C/C=C/C=C(C)/C=C/C=C(C)/C(O[C@@H]1O[C@@H]([C@H]([C@@H]([C@H]1O)O)O)CO)=O)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
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Alzheimer’s Disease Modeling
Alzheimer’s Disease (AD) is a neurodegenerative disorder characterized by a progressive decline in cognitive functions and loss of specific types of neurons and synapses. Alzheimer's symptoms can be simulated in mice by injecting drugs (such as Aβ) or genetically modified.
Purity & Documentation
References
[1]. Chalatsa I, et, al. The Crocus sativus Compounds trans-Crocin 4 and trans-Crocetin Modulate the Amyloidogenic Pathway and Tau Misprocessing in Alzheimer Disease Neuronal Cell Culture Models. Front Neurosci. 2019 Mar 26;13:249. [Content Brief]
[2]. Koulakiotis NS, et, al. Crocus-derived compounds alter the aggregation pathway of Alzheimer's Disease: associated beta amyloid protein. Sci Rep. 2020 Oct 23;10(1):18150. [Content Brief]
[3]. Karkoula E, et, al. Trans-crocin 4 is not hydrolyzed to crocetin following i.p. administration in mice, while it shows penetration through the blood brain barrier. Fitoterapia. 2018 Sep;129:62-72. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Crocin-4 Related Classifications
HY-N10183 Related Classifications
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