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  3. Cyanine 5 Tyramide

Cyanine 5 Tyramide  (Synonyms: Tyramide-Cy5)

Cat. No.: HY-136247 Purity: 95.04%
Handling Instructions Technical Support

Cyanine 5 Tyramide (Tyramide-Cy5), a red fluorescent dye, is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids.

For research use only. We do not sell to patients.

Cyanine 5 Tyramide

Cyanine 5 Tyramide Chemical Structure

CAS No. : 1431148-26-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
1 mg In-stock
5 mg In-stock
10 mg In-stock
50 mg   Get quote  
100 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

Other Forms of Cyanine 5 Tyramide:

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Description

Cyanine 5 Tyramide (Tyramide-Cy5), a red fluorescent dye, is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids[1].

Molecular Weight

775.97

Formula

C41H49N3O8S2

CAS No.
Appearance

Solid

Color

Purple to purplish red

Emission (Em)

670

Excitation (Ex)

645

SMILES

O=S(C1=CC2=C([N+](CC)=C(/C=C/C=C/C=C3N(CCCCCC(NCCC4=CC=C(O)C=C4)=O)C5=C(C=C(S(=O)(O)=O)C=C5)C/3(C)C)C2(C)C)C=C1)([O-])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (80.54 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2887 mL 6.4435 mL 12.8871 mL
5 mM 0.2577 mL 1.2887 mL 2.5774 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.2887 mL 6.4435 mL 12.8871 mL 32.2177 mL
5 mM 0.2577 mL 1.2887 mL 2.5774 mL 6.4435 mL
10 mM 0.1289 mL 0.6444 mL 1.2887 mL 3.2218 mL
15 mM 0.0859 mL 0.4296 mL 0.8591 mL 2.1478 mL
20 mM 0.0644 mL 0.3222 mL 0.6444 mL 1.6109 mL
25 mM 0.0515 mL 0.2577 mL 0.5155 mL 1.2887 mL
30 mM 0.0430 mL 0.2148 mL 0.4296 mL 1.0739 mL
40 mM 0.0322 mL 0.1611 mL 0.3222 mL 0.8054 mL
50 mM 0.0258 mL 0.1289 mL 0.2577 mL 0.6444 mL
60 mM 0.0215 mL 0.1074 mL 0.2148 mL 0.5370 mL
80 mM 0.0161 mL 0.0805 mL 0.1611 mL 0.4027 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Cyanine 5 Tyramide
Cat. No.:
HY-136247
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