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CYT296 is a target chromatin de-condensation compound. CYT296 can improve the induction of induced pluripotent stem cell (iPSCs) mediated by defined factors (OSKM) and induce an open chromatin state in Mouse Embryonic Fibroblast (MEFs) to facilitate somatic cell reprogramming. CYT296 can be used for cell replacement therapies and drug screening research.

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CYT296 Chemical Structure

CYT296 Chemical Structure

CAS No. : 1799392-31-6

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1 mg USD 90 In-stock
5 mg USD 209 In-stock
10 mg USD 335 In-stock
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50 mg USD 1128 In-stock
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Based on 1 publication(s) in Google Scholar

CYT296 Related Antibodies

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Description

CYT296 is a target chromatin de-condensation compound. CYT296 can improve the induction of induced pluripotent stem cell (iPSCs) mediated by defined factors (OSKM) and induce an open chromatin state in Mouse Embryonic Fibroblast (MEFs) to facilitate somatic cell reprogramming. CYT296 can be used for cell replacement therapies and drug screening research[1].

In Vitro

CYT296 (250nM, 72 h) changes the MEF chromatin and allows easier access of Yamanaka factors and facilitates the reprogramming process[1].
CYT296 (250nM, 72 h) block heterochromatin assembly of MEFs to improve the generation of iPSC[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CYT296 Related Antibodies

Immunofluorescence[1]

Cell Line: MEFs
Concentration: 250 nM
Incubation Time: 72 h
Result: Reduced the number and total intensity of HP1α+ and H3K9me3+ foci in the nuclei of MEFs.

Western Blot Analysis[1]

Cell Line: MEFs
Concentration: 250 nM
Incubation Time: 72 h
Result: Speeded up the expression of Nanog during 4F-mediated reprogramming and appeared as early as day 8.
Reduced the protein level of HP1α during reprogramming.
In Vivo

CYT296 (10-15 4F- and 2F -iPSC clones for subcutaneous injection, once) generates the iPSCs are pluripotent in the NOD-SCID mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 4F- and 2F-iPSC clones mice[1]
Dosage: 10-15 4F- and 2F -iPSC clones
Administration: Subcutaneous injection (s.c.)
Result: Obtained 6 out of 10 born mice from clone 4F-1 and 9 out of 15 born mice from clone 2F-1 were chimeras.
Molecular Weight

296.38

Formula

C18H16O2S
CAS No.
Appearance

Oil

Color

White to off-white

SMILES

O=C1[C@@]2(CSC3=CC=CC=C31)[C@H](C4=CC=C(C=C4)OC)C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (337.40 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3740 mL 16.8702 mL 33.7405 mL
5 mM 0.6748 mL 3.3740 mL 6.7481 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (8.44 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (8.44 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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mg/kg

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(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3740 mL 16.8702 mL 33.7405 mL 84.3512 mL
5 mM 0.6748 mL 3.3740 mL 6.7481 mL 16.8702 mL
10 mM 0.3374 mL 1.6870 mL 3.3740 mL 8.4351 mL
15 mM 0.2249 mL 1.1247 mL 2.2494 mL 5.6234 mL
20 mM 0.1687 mL 0.8435 mL 1.6870 mL 4.2176 mL
25 mM 0.1350 mL 0.6748 mL 1.3496 mL 3.3740 mL
30 mM 0.1125 mL 0.5623 mL 1.1247 mL 2.8117 mL
40 mM 0.0844 mL 0.4218 mL 0.8435 mL 2.1088 mL
50 mM 0.0675 mL 0.3374 mL 0.6748 mL 1.6870 mL
60 mM 0.0562 mL 0.2812 mL 0.5623 mL 1.4059 mL
80 mM 0.0422 mL 0.2109 mL 0.4218 mL 1.0544 mL
100 mM 0.0337 mL 0.1687 mL 0.3374 mL 0.8435 mL
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CYT296 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CYT2961799392-31-6CYT 296CYT-296Otherssmall molecule compoundchromatin remodelingMEFsInhibitorinhibitorinhibit

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