Cytarabine 5’-monophosphate
Based on 1 Customer Validation
Cytarabine 5′-monophosphate is a metabolite of the nucleoside analog Cytarabine (HY-13605), catalyzed by deoxycytidine kinase. Cytarabine 5′-monophosphate is incorporated into DNA by DNA polymerase α, which reduces the rate of DNA synthesis. At a concentration of 15 mM, Cytarabine 5′-monophosphate inhibits nuclear and mitochondrial DNA replication in Saccharomyces cerevisiae (S. cerevisiae). Additionally, Cytarabine 5′-monophosphate (3.5-75.1 mg/kg) improves survival in leukemia mice (L1210 mice). Cytarabine 5′-monophosphate can be used in cancer research.
For research use only. We do not sell to patients.
- Purity: 98.04%
- CAS No.: 7075-11-8
- Formula: C9H14N3O8P
- Molecular Weight:323.20
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Storage:
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All DNA/RNA Synthesis Isoforms
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Biological Activity
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DNA Polymerase |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| L1210 | ED50 |
0.005 μM
Compound: ara-CMP
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Cytotoxicity against mouse L1210 cells assessed as growth inhibition measured at 72 hrs in presence of 100 uM prednisolone by trypan blue exclusion assay
Cytotoxicity against mouse L1210 cells assessed as growth inhibition measured at 72 hrs in presence of 100 uM prednisolone by trypan blue exclusion assay
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[PMID: 118258] |
| L1210 | ED50 |
0.01 μM
Compound: ara-CMP
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Cytotoxicity against mouse L1210 cells assessed as growth inhibition measured at 72 hrs in presence of 5 uM prednisolone by trypan blue exclusion assay
Cytotoxicity against mouse L1210 cells assessed as growth inhibition measured at 72 hrs in presence of 5 uM prednisolone by trypan blue exclusion assay
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[PMID: 118258] |
| L1210 | ED50 |
0.05 μM
Compound: ara-CMP
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Cytotoxicity against mouse L1210 cells assessed as growth inhibition measured at 72 hrs by trypan blue exclusion assay
Cytotoxicity against mouse L1210 cells assessed as growth inhibition measured at 72 hrs by trypan blue exclusion assay
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[PMID: 118258] |
Chemical Information
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CAS No. 7075-11-8
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Appearance Solid
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Molecular Weight 323.20
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Formula C9H14N3O8P
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Color White to off-white
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SMILES
O[C@H]1[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]1N2C(N=C(N)C=C2)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
H2O : 4.17 mg/mL (12.90 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
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Handling Instructions (2659 KB)
References
[1]. Momparler RL, et al. Mammalian deoxynucleoside kinase. I. Deoxycytidine kinase: purification, properties, and kinetic studies with cytosine arabinoside. J Biol Chem. 1968 Aug 25;243(16):4298-304. [Content Brief]
[2]. Huang S, et al. Development and application of a rapid and sensitive liquid chromatography-mass spectrometry method for simultaneous analysis of cytarabine, cytarabine monophosphate, cytarabine diphosphate and cytarabine triphosphate in the cytosol and nucleus. J Pharm Biomed Anal. 2022 Mar 20;211:114582. [Content Brief]
[3]. Perrino FW, et al. Incorporation of cytosine arabinoside monophosphate into DNA at internucleotide linkages by human DNA polymerase alpha. J Biol Chem. 1992 Nov 15;267(32):23043-51. [Content Brief]
[4]. McIntosh EM, et al. Inhibition of DNA replication in Saccharomyces cerevisiae by araCMP. Curr Genet. 1986;10(8):579-85. [Content Brief]
[5]. Schrecker AW, et al. Antitumor effect and mode of action of 1-beta-D-arabinofuranosylcytosine 5'-phosphate in leukemia L1210. Cancer Res. 1968 Apr;28(4):802-3. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 3.0941 mL | 15.4703 mL | 30.9406 mL | 77.3515 mL |
| 5 mM | 0.6188 mL | 3.0941 mL | 6.1881 mL | 15.4703 mL | |
| 10 mM | 0.3094 mL | 1.5470 mL | 3.0941 mL | 7.7351 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Cytarabine 5’-monophosphate Related Classifications
HY-W344074 Related Classifications
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