α-Carotene
Based on 1 Customer Validation
α-Carotene, a precursor of vitamin A, is used as an anti-metastatic agent or as an adjuvant for anti-cancer agents. α-Carotene is isolated from yellow-orange and dark-green vegetables.
For research use only. We do not sell to patients.
- Purity: 95.0%
- CAS No.: 7488-99-5
- Formula: C40H56
- Molecular Weight:536.87
-
Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
α-Carotene (0.5-2.5 μM; 24 hours) significantly increases protein expression of TIMP-1 and TIMP-2 in a concentration-dependent manner in LLC cells. AC (0.5-2.5 μM) significantly increases PAI-1 protein expression. α-Carotene (2.5 μM) also significantly inhibits integrin β1-mediated phosphorylation of focal adhesion kinase (FAK) which then decreased the phosphorylation of MAPK family[2].
α-Carotene (0.5, 1, 2.5 μM; 48 hours) significantly and concentration-dependently inhibits invasion of LLC during 48 h of incubation[2].
α-Carotene (0.5, 1, 2.5 μM; 24 hours) significantly decreases activities of MMP-9, -2 and uPA in concentration-dependent manner in LLC cells[2].
α-Carotene (2, 5, 10 μM; 7 days) inhibits the proliferation of the human neuroblastoma cell line GOTO in a dose- and time-dependent manner. α-Carotene (5 μM; 48 hours) halts the cell cycle at the G0/G1 phase concomitantly with a reduction in the mRNA expression of the protooncogene N-Myc[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:Lewis lung carcinoma (LLC) cells
-
Concentration:0.5, 1, 2.5 μM
-
Incubation Time:24 hours
-
Result:Significantly increased protein expression of TIMP-1 and TIMP-2 in a concentration-dependent manner in LLC cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:C57BL/6 male mice (4 weeks old; 20-25 g) with LLC cells[2]
-
Dosage:5 mg/kg
-
Administration:Oral; twice a week; for additional 3 weeks
-
Result:Significantly decreased lung metastasis.
Chemical Information
-
CAS No. 7488-99-5
-
Appearance Solid
-
Molecular Weight 536.87
-
Formula C40H56
-
Color Red to reddish brown
-
SMILES
CC1(C)[C@@H](/C=C/C(C)=C/C=C/C(C)=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C2=C(C)CCCC2(C)C)C(C)=CCC1
-
Structure Classification
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 1 mg/mL (1.86 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (271 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
[1]. Bushway, R.J., et al. Determination of α- and β-carotene in fruit and vegetables by high performance liquid chromatography. Can. Inst. Food Sci. Technol. J. 15(3), 165-169 (1982). [Content Brief]
[2]. Liu YZ, et al. Alpha-carotene inhibits metastasis in Lewis lung carcinoma in vitro, and suppresses lungmetastasis and tumor growth in combination with taxol in tumor xenografted C57BL/6 mice. J Nutr Biochem. 2015 Jun;26(6):607-15. [Content Brief]
[3]. Murakoshi M, et al. Inhibitory effects of alpha-carotene on proliferation of the human neuroblastoma cell line GOTO. J Natl Cancer Inst. 1989 Nov 1;81(21):1649-52. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8626 mL | 9.3132 mL | 18.6265 mL | 46.5662 mL |
Powered by Bioz