D-3
Based on 1 Customer Validation
D-3, a phosphorpeptide, is an efficient, simple, and specific iPSC-eliminating agent.
For research use only. We do not sell to patients.
- Purity: 99.21%
- CAS No.: 1967815-98-0
- Formula: C48H47N4O10P
- Molecular Weight:870.88
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Storage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
D-3 prevents residual iPSC-induced teratoma formation in a mouse tumorigenicity assay[1].
D-3 induces obvious loss of viability in 201B7 cells, with half maximal inhibitory concentration value of 192.3 ± 57.4 μM[1].
D-3 induces an increase in the amount of activated elF2a, p38, and p44/42 MAPK, which
are activated in response to cellular stress. D-3 also increases the number of Annexin-V and SYTOX-positive cells, indicating apoptotic and dead cells after D-3 treatment[1].
D-3 has little influence on various non-iPSCs, including hepatocytes and neurons[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Six human iPSCs lines and one human ESC line (khES1).
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Concentration:400 μM.
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Incubation Time:1-2 h.
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Result:Sufficient to induce a viability loss (>99% in all iPSC lines and >95% in khES1).
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 1967815-98-0
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Appearance Solid
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Molecular Weight 870.88
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Formula C48H47N4O10P
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Color White to off-white
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Solvent & Solubility
H2O : ≥ 53.3 mg/mL (61.20 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.1483 mL | 5.7413 mL | 11.4826 mL | 28.7066 mL |
| 5 mM | 0.2297 mL | 1.1483 mL | 2.2965 mL | 5.7413 mL | |
| 10 mM | 0.1148 mL | 0.5741 mL | 1.1483 mL | 2.8707 mL | |
| 15 mM | 0.0766 mL | 0.3828 mL | 0.7655 mL | 1.9138 mL | |
| 20 mM | 0.0574 mL | 0.2871 mL | 0.5741 mL | 1.4353 mL | |
| 25 mM | 0.0459 mL | 0.2297 mL | 0.4593 mL | 1.1483 mL | |
| 30 mM | 0.0383 mL | 0.1914 mL | 0.3828 mL | 0.9569 mL | |
| 40 mM | 0.0287 mL | 0.1435 mL | 0.2871 mL | 0.7177 mL | |
| 50 mM | 0.0230 mL | 0.1148 mL | 0.2297 mL | 0.5741 mL | |
| 60 mM | 0.0191 mL | 0.0957 mL | 0.1914 mL | 0.4784 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
D-3 Related Classifications
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