D-Glucono-1,4-lactone
Based on 1 Customer Validation
D-Glucono-1,4-lactone is a derivative of D-gluconic acid (HY-Y0569C). D-Glucono-1,4-lactone can be isolated from fruits, vegetables, honey, kombucha and wine. D-Glucono-1,4-lactone inhibits thrombin-induced arachidonic acid peroxidation and platelet activation. D-Glucono-1,4-lactone reduces superoxide anion production in thrombin-activated platelets. D-Glucono-1,4-lactone exerts antioxidant activity on platelets under oxidative stress conditions and prevents excessive platelet activation through antioxidant mechanisms. D-Glucono-1,4-lactone can be used in research related to cardiovascular diseases.
For research use only. We do not sell to patients.
- CAS No.: 1198-69-2
- Formula: C6H10O6
- Molecular Weight:178.14
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
D-Glucono-1,4-lactone (0.5-3 mM; 5 min) dose-dependently inhibits thrombin-induced arachidonic acid peroxidation in washed platelets, an effect measured by reduced TBARS levels, with significant effects observed at concentrations ranging from 0.5 to 3 mM[1].
D-Glucono-1,4-lactone (0.5-3 mM; 5 min) significantly inhibits thrombin-induced superoxide anion production in washed platelets[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1198-69-2
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Appearance Solid
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Molecular Weight 178.14
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Formula C6H10O6
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Color White to off-white
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SMILES
OC[C@H]([C@]1([H])[C@@H]([C@H](C(O1)=O)O)O)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (561.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.6136 mL | 28.0678 mL | 56.1356 mL | 140.3391 mL |
| 5 mM | 1.1227 mL | 5.6136 mL | 11.2271 mL | 28.0678 mL | |
| 10 mM | 0.5614 mL | 2.8068 mL | 5.6136 mL | 14.0339 mL | |
| 15 mM | 0.3742 mL | 1.8712 mL | 3.7424 mL | 9.3559 mL | |
| 20 mM | 0.2807 mL | 1.4034 mL | 2.8068 mL | 7.0170 mL | |
| 25 mM | 0.2245 mL | 1.1227 mL | 2.2454 mL | 5.6136 mL | |
| 30 mM | 0.1871 mL | 0.9356 mL | 1.8712 mL | 4.6780 mL | |
| 40 mM | 0.1403 mL | 0.7017 mL | 1.4034 mL | 3.5085 mL | |
| 50 mM | 0.1123 mL | 0.5614 mL | 1.1227 mL | 2.8068 mL | |
| 60 mM | 0.0936 mL | 0.4678 mL | 0.9356 mL | 2.3390 mL | |
| 80 mM | 0.0702 mL | 0.3508 mL | 0.7017 mL | 1.7542 mL | |
| 100 mM | 0.0561 mL | 0.2807 mL | 0.5614 mL | 1.4034 mL |
D-Glucono-1,4-lactone Related Classifications
HY-W047191 Related Classifications
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