DA 3003-2
Based on 1 Customer Validation
DA 3003-2 is a potent and selectively Cdc25 inhibitor. DA 3003-2 shows antiproliferative activity. DA 3003-2 induces cell cycle arrest at the G2/M phase and increases the expression of P-tyr15 Cdc2. DA 3003-2 has the potential for the research of prostate cancer.
For research use only. We do not sell to patients.
- Purity : 98.36%
- CAS No.: 383907-47-9
- Formula: C15H16ClN3O3
- Molecular Weight:321.76
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CCD-841CoN | IC50 |
>2 μM
Compound: 6b
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Cytotoxicity against human CCD-841CoN cells assessed as growth inhibition after 72 hrs by MTS assay
Cytotoxicity against human CCD-841CoN cells assessed as growth inhibition after 72 hrs by MTS assay
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[PMID: 37302341] |
| DLD-1 | IC50 |
0.59 μM
Compound: 6b
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Cytotoxicity against human DLD1 cells assessed as growth inhibition after 72 hrs by MTS assay
Cytotoxicity against human DLD1 cells assessed as growth inhibition after 72 hrs by MTS assay
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[PMID: 37302341] |
| HEL 299 | IC50 |
>2 μM
Compound: 6b
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Cytotoxicity against human HEL 299 cells assessed as growth inhibition after 72 hrs by MTS assay
Cytotoxicity against human HEL 299 cells assessed as growth inhibition after 72 hrs by MTS assay
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[PMID: 37302341] |
| MDA-MB-231 | IC50 |
0.44 μM
Compound: 6b
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Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTS assay
Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTS assay
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[PMID: 37302341] |
| MRC5 | IC50 |
>2 μM
Compound: 6b
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Cytotoxicity against human MRC5 cells assessed as growth inhibition after 72 hrs by MTS assay
Cytotoxicity against human MRC5 cells assessed as growth inhibition after 72 hrs by MTS assay
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[PMID: 37302341] |
In Vitro
DA 3003-2 (0.3-30 µM; 48 h) shows antiproliferative activity with an IC50 value of 5 µM in PC-3 cells[1].
DA 3003-2 (5, 10 µM; 24, 1 h) induces cell cycle arrest at G2/M phase and increases the expression of P-tyr15 Cdc2 in PC-3 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:PC-3 cells
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Concentration:0.3-30 µM
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Incubation Time:48 h
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Result:Showed antiproliferative efficacy in a dose-dependent manner with an IC50 value of 5 µM.
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Cell Line:PC-3 cells
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Concentration:5, 10 µM
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Incubation Time:24 h
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Result:Induced cell cycle arrest at G2/M phase.
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Cell Line:PC-3 cells
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Concentration:5, 10 µM
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Incubation Time:1 h
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Result:Increased the expression of P-tyr15 Cdc2.
Chemical Information
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CAS No. 383907-47-9
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Appearance Solid
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Molecular Weight 321.76
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Formula C15H16ClN3O3
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Color Brown to red
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SMILES
O=C(C1=C(C2=O)N=CC=C1)C(NCCN3CCOCC3)=C2Cl
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Synonyms
NSC663285
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
In Vitro:
DMSO : 150 mg/mL (466.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 3.75 mg/mL (11.65 mM); Clear solution
This protocol yields a clear solution of ≥ 3.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Flow cytometric DNA-content cell-cycle staining
Flow cytometric DNA-content cell-cycle staining measures the fluorescence intensity of DNA-bound fluorochromes in single cells or nuclei to estimate DNA content distributions, allowing assignment of populations to G0/G1, S, and G2/M phases by DNA histogram deconvolution. Propidium iodide (PI) intercalates into DNA, and PI fluorescence is proportional to cellular DNA content when staining is performed under conditions that make DNA accessible and minimize non-DNA signal. Cells with G2/M DNA content are expected to show approximately twice the fluorescence intensity of G0/G1 cells, while S-phase cells occupy intermediate fluorescence values. PI-based DNA-content analysis can also detect cells with fractional DNA content, often reported as sub-G1, when DNA fragmentation and extraction during staining reduce retained DNA signal in apoptotic cells. DAPI is an alternative DNA fluorochrome for univariate DNA-content analysis, while bivariate approaches combining DNA content with proliferation
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BrdU Incorporation Assay
Bromodeoxyuridine (BrdU) incorporation assay is based on the principle that BrdU, a thymidine analog, is incorporated into newly synthesized DNA during the S phase of the cell cycle, thereby serving as a marker of DNA replication and cellular proliferation. Incorporated BrdU can be detected using anti-BrdU antibodies following DNA denaturation, enabling visualization or quantification of proliferating cells through immunochemical detection methods such as immunofluorescence or immunohistochemistry.
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Protocol for Cell Cycle
Cell-cycle analysis by flow cytometry measures DNA content in single cells to estimate the fraction of cells in G0/G1, S, and G2/M phases. Propidium iodide intercalates into DNA, and after RNA removal with RNase, fluorescence intensity reflects cellular DNA content: 2N cells are assigned to G0/G1, cells between 2N and 4N to S phase, and 4N cells to G2/M. DNA-content analysis alone cannot reliably separate G0 from G1 or G2 from M. Ki-67 can distinguish quiescent G0 cells from cycling cells, EdU or BrdU incorporation marks active DNA synthesis in S phase, and phospho-histone H3 staining identifies mitotic cells within the 4N population.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1079 mL | 15.5395 mL | 31.0791 mL | 77.6977 mL |
| 5 mM | 0.6216 mL | 3.1079 mL | 6.2158 mL | 15.5395 mL | |
| 10 mM | 0.3108 mL | 1.5540 mL | 3.1079 mL | 7.7698 mL | |
| 15 mM | 0.2072 mL | 1.0360 mL | 2.0719 mL | 5.1798 mL | |
| 20 mM | 0.1554 mL | 0.7770 mL | 1.5540 mL | 3.8849 mL | |
| 25 mM | 0.1243 mL | 0.6216 mL | 1.2432 mL | 3.1079 mL | |
| 30 mM | 0.1036 mL | 0.5180 mL | 1.0360 mL | 2.5899 mL | |
| 40 mM | 0.0777 mL | 0.3885 mL | 0.7770 mL | 1.9424 mL | |
| 50 mM | 0.0622 mL | 0.3108 mL | 0.6216 mL | 1.5540 mL | |
| 60 mM | 0.0518 mL | 0.2590 mL | 0.5180 mL | 1.2950 mL | |
| 80 mM | 0.0388 mL | 0.1942 mL | 0.3885 mL | 0.9712 mL | |
| 100 mM | 0.0311 mL | 0.1554 mL | 0.3108 mL | 0.7770 mL |
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