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Cat. No.: HY-P2628

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Dabcyl-YVADAPV-EDANS

Dabcyl-YVADAPV-EDANS is a fluorogenic interleukin-1β converting enzyme (ICE) substrate for measuring ICE-like protease activity. Dabcyl-YVADAPV-EDANS detects fluorescence at 360 nm excitation and 480 nm emission wavelengths. ICE-like protease is a critical mediator of K⁺ deprivation-induced apoptosis of cerebellar granule neurons.

For research use only. We do not sell to patients.

CAS No.: 161877-70-9
Formula: C₆₁H₇₆N₁₂O₁₄S
Molecular Weight:1233.39

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Dabcyl-YVADAPV-EDANS Chemical Structure

CAS No.: 161877-70-9

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
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Biological Activity

Description

In Vitro

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
ICE activity assay methods^[1]:
(1) Cerebellar granule neurons were seeded in 24-well plates at a density of 2.1*10⁵ cells/cm².
(2) After the switch to medium containing low (5 mM) or high (25 mM) concentrations of KCl at DIV 8, cerebellar granule neurons were washed with Locke’s buffer and made permeable by 0.03% Digitonin (HY-N4000).
(3) After 10 min, DABCYL-YVADAPV-EDANS (20 μM) was added.
(4) Fluorescence was determined in 10 min intervals for 1 h using 360 nm excitation and 480 nm emission wavelengths in the 24-well plates.
(5) The fluorometric intensity peaked at 20 min. This time point was used for statistical analysis.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 161877-70-9
Molecular Weight 1233.39
Formula C₆₁H₇₆N₁₂O₁₄S
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (258 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Schulz JB, et al. Potassium deprivation-induced apoptosis of cerebellar granule neurons: a sequential requirement for new mRNA and protein synthesis, ICE-like protease activity, and reactive oxygen species. J Neurosci. 1996 Aug 1;16(15):4696-706. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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