Art. -Nr.: HY-111675

Ac-FLTD-CMK

Name: Ac-FLTD-CMK
Brand: MedChemExpress
SKU: HY-111675
Price: 370.00 USD
Availability: InStock
Rating: 5 (12 reviews)

12 Cited Publications

Kundenbewertung

Based on 12 publication(s) in Google Scholar

Ac-FLTD-CMK, a gasdermin D (GSDMD)-derived inhibitor, is a specific inflammatory caspases inhibitor. Ac-FLTD-CMK is effective against caspases-1 (IC₅₀ of 46.7 nM), caspases-4 (IC₅₀ of 1.49 μM), caspases-5 (IC₅₀ of 329 nM), and caspases-11 , but not the apoptotic caspases such as caspase-3.

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Reinheit : 99.18%
CAS. Nr.: 2376255-48-8
Formel: C₂₆H₃₇ClN₄O₈
Molecular Weight:569.05

Speicherung:

-20°C, protect from light, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Ac-FLTD-CMK Chemical Structure

CAS. Nr.: 2376255-48-8

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (12)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

^[1]

Caspase-1

46.7 nM (IC₅₀)

Caspase-4

1.49 μM (IC₅₀)

Caspase-5

329 nM (IC₅₀)

Caspase-11

Alle Caspase Isoform-spezifische Produkte anzeigen :

In Vitro

Ac-FLTD-CMK, inhibits GSDMD cleavage by caspases-1, -4, -5, and -11 in vitro, suppresses pyroptosis downstream of both canonical and noncanonical inflammasomes, as well as reduces IL-1β release following activation of the NLRP3 inflammasome in macrophages. By contrast, Ac-FLTD-CMK does not target caspase-3 or apoptotic cell death. Crystal structure of caspase-1 in complex with Ac-FLTD-CMK reveals extensive enzyme-inhibitor interactions involving both hydrogen bonds and hydrophobic contacts^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 2376255-48-8
Appearance Solid
Molecular Weight 569.05
Formel C₂₆H₃₇ClN₄O₈
Color White to off-white
SMILES

ClCC([C@H](CC(O)=O)NC([C@H]([C@H](O)C)NC([C@H](CC(C)C)NC([C@@H](NC(C)=O)CC1=CC=CC=C1)=O)=O)=O)=O
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, protect from light, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Publications (12)

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Journal Impact Factor

Cell

Small-molecule GSDMD agonism in tumors stimulates antitumor immunity without toxicity. [Abstract]

2024 Oct 31;187(22):6165-6181.e22. PMID: 39243763
Cell Rep Med

HIF-activated priming of TRAIL-induced cell death determines epigenetic vulnerability in kidney cancer. [Abstract]

2026 Feb 18:102630. PMID: 41713410
Cell Death Differ

RIPK3 sequentially recruits MLKL and RIPK1 to induce PANoptosis and chemokine production. [Abstract]

2026 Apr 4. PMID: 41935203
Cell Death Dis

Complement induces podocyte pyroptosis in membranous nephropathy by mediating mitochondrial dysfunction. [Abstract]

2022 Mar 29;13(3):281. PMID: 35351877
Part Fibre Toxicol

Exposure to high dose of polystyrene nanoplastics causes trophoblast cell apoptosis and induces miscarriage. [Abstract]

2024 Mar 7;21(1):13. PMID: 38454452
Int J Biol Macromol

2026 May 29;370:152795.
Cell Regen

METTL3 restricts RIPK1-dependent cell death via the ATF3-cFLIP axis in the intestinal epithelium. [Abstract]

2024 Aug 2;13(1):14. PMID: 39093347
Chem Biol Interact

A novel non-nad-based PARP1 inhibitor, Japoflavone B, triggered Caspase-3/GSDME-mediated pyroptosis through ROS/p38/p53 pathway in NSCLC. [Abstract]

2025 Oct 22:420:111693. PMID: 40754060
Neurochem Res

HAT1 Protects Against Cerebral Ischemia Injury by Inhibiting TFRC-Mediated Ferroptosis. [Abstract]

2025 Dec 29;51(1):24. PMID: 41460540
Mol Divers

Proteochemometrics modeling for prediction of the interactions between caspase isoforms and their inhibitors. [Abstract]

2023 Feb;27(1):249-261. PMID: 35438428
Toxicol Appl Pharmacol

Chrysin enhances sunitinib sensitivity in renal cell carcinoma by inducing ferroptosis via targeting PI3K/Akt/GPX4 pathway. [Abstract]

2025 Aug 21:117531. PMID: 40848919
Biomed Pharmacother

The pyroptosis mechanism of ototoxicity caused by unconjugated bilirubin in neonatal hyperbilirubinemia. [Abstract]

2023 Sep:165:115162. PMID: 37467648

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (175.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.7573 mL

8.7866 mL

17.5731 mL

5 mM

0.3515 mL

1.7573 mL

3.5146 mL

10 mM

0.1757 mL

0.8787 mL

1.7573 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.39 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (4.39 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.39 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Jie Yang, et al. Mechanism of gasdermin D recognition by inflammatory caspases and their inhibition by a gasdermin D-derived peptide inhibitor. Proc Natl Acad Sci U S A. 2018 Jun 26;115(26):6792-6797. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.7573 mL	8.7866 mL	17.5731 mL	43.9329 mL
	5 mM	0.3515 mL	1.7573 mL	3.5146 mL	8.7866 mL
	10 mM	0.1757 mL	0.8787 mL	1.7573 mL	4.3933 mL
	15 mM	0.1172 mL	0.5858 mL	1.1715 mL	2.9289 mL
	20 mM	0.0879 mL	0.4393 mL	0.8787 mL	2.1966 mL
	25 mM	0.0703 mL	0.3515 mL	0.7029 mL	1.7573 mL
	30 mM	0.0586 mL	0.2929 mL	0.5858 mL	1.4644 mL
	40 mM	0.0439 mL	0.2197 mL	0.4393 mL	1.0983 mL
	50 mM	0.0351 mL	0.1757 mL	0.3515 mL	0.8787 mL
	60 mM	0.0293 mL	0.1464 mL	0.2929 mL	0.7322 mL
	80 mM	0.0220 mL	0.1098 mL	0.2197 mL	0.5492 mL
	100 mM	0.0176 mL	0.0879 mL	0.1757 mL	0.4393 mL

Bioz

Keywords

Ac-FLTD-CMK

12 Cited Publications

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Ac-FLTD-CMK

Publications Citing Use of MedChemExpress (MCE) Ac-FLTD-CMK

Alle Caspase Isoform-spezifische Produkte anzeigen