Dammarenediol II

Cat. No.: HY-N3677 Purity: 99.81%: Data Sheet
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Dammarenediol II is a ginsenoside precursor. Dammarenediol II reduces the activity of O-GlcNAc transferase (OGT) and downregulates the global O-GlcNAcylation level. Dammarenediol II inhibits the phosphorylation of Akt, mTOR and GSK3β. Dammarenediol II inhibits human carboxylesterase activity, VEGF-induced ROS production, stress fiber formation and vascular endothelial cadherin disruption. Dammarenediol II promotes cell apoptosis (apoptosis), increases the levels of cleaved PARP1 and p53, and inhibits retinal microvascular leakage. Dammarenediol II can be used in studies related to liver cancer and diabetic retinopathy.

For research use only. We do not sell to patients.

Dammarenediol II Chemical Structure

CAS No. : 14351-29-2

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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•Related Screening Libraries:

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Akt Akt1 Akt2 Akt3

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mTOR mTORC1 mTORC2

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GSK-3 GSK-3α GSK-3β

View All PARP Isoform Specific Products:

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PARP PARP1 PARP2 PARP3 PARP4 TNKS1/PARP5A TNKS2/PARP5B PARP7 PARP10 PARP14 PARP15 PARP12 PARP16

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
Vero	IC₅₀	7 μg/mL Compound: 2, dammarenediol-II	Antiviral activity against HSV1 MacIntyre replication in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect after 48 hrs Antiviral activity against HSV1 MacIntyre replication in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect after 48 hrs	[PMID: 2828553]
Vero	IC₅₀	7 μg/mL Compound: 2, dammarenediol-II	Antiviral activity against HSV2 MS replication infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect after 48 hrs Antiviral activity against HSV2 MS replication infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect after 48 hrs	[PMID: 2828553]

In Vitro

Dammarenediol II (40 μM; 24 h) reduces O-GlcNAc levels in HepG2, Huh7, SK-HEP1, SNU-182 and SNU-387 cells^[1].
Dammarenediol II (0.01-2.0 μg/mL; 30 min pretreatment, 10 min VEGF incubation) dose-dependently inhibits VEGF-induced intracellular ROS production in human umbilical vein endothelial cells, with the maximum inhibitory effect observed at 1.0 μg/mL^[2].
Dammarenediol II (1 μg/mL; 30 min, 1 h VEGF) inhibits VEGF-induced stress fiber formation in human umbilical vein endothelial cells^[2].
Dammarenediol II (1 μg/mL; 30 min preincubation, 90 min VEGF treatment) inhibits VEGF-induced VE-cadherin disruption and maintains the integrity of adherens junctions in human umbilical vein endothelial cells^[2].
Dammarenediol II (0-50 μM; 24 h) significantly reduces the viability of HepG2 cells at 50 μM^[1].
Dammarenediol II (0-40 μM; 24 h) significantly downregulates the expression level of OGT in HepG2 cells^[1].
Dammarenediol II (0-40 μM; 24 h) reduces the EC₅₀ of Etoposide (HY-13629) for regulating caspase-3/7 activity from 17.69 μM to 4.86 μM in HepG2 cells, upregulates the expression levels of cleaved-PARP1 and p53 proteins, induces apoptosis in HepG2, Huh7, SK-HEP1, SNU-182 and SNU-387 cells, and synergistically enhances the anticancer activity of Etoposide^[1].
Dammarenediol II (40 μM; 24 h) inhibits the phosphorylation levels of Akt, GSK3β and mTOR in HepG2 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	Huh7, SK-HEP1, SNU-182, SNU-387 human liver carcinoma cells
Concentration:	40 μM
Incubation Time:	24 h
Result:	Reduced O-GlcNAc levels in all tested human liver carcinoma cell lines: Huh7, SK-HEP1, SNU-182, and SNU-387.

Cell Viability Assay^[1]

Cell Line:	HepG2 cells
Concentration:	0, 2.5, 5, 10, 20, 40, 50 μM
Incubation Time:	24 h
Result:	Significantly reduced the viability of HepG2 cells at 50 μM

Immunofluorescence^[1]

Cell Line:	HepG2 cells
Concentration:	0, 10, 20, 40 μM + 20 μM Etoposide
Incubation Time:	24 h
Result:	Significantly increased the proportion of TUNEL-positive cells in a dose-dependent manner.

In Vivo

Dammarenediol-II (2.5 μg; intravitreal; single injection) prevents retinal microvascular leakage in diabetic mice, reducing FITC-dextran fluorescence intensity to control levels^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 (6-week-old male; diabetic via single intraperitoneal injection of streptozotocin)^[2]
Dosage:	2.5 μg
Administration:	intravitreal; single injection
Result:	Completely blocked marked FITC-dextran extravasation in diabetic mouse retinas. Reduced average FITC-dextran fluorescence intensity in diabetic mouse retinas to levels comparable to control mice (intensity was ~2-fold higher in untreated diabetic mice than controls).

Molecular Weight

444.73

Formula

C₃₀H₅₂O₂

CAS No.

14351-29-2

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]1(CC[C@@]2([H])C3(C)C)[C@@](CC[C@@]4([H])[C@]1(CC[C@]4([H])[C@@](C)(O)CC/C=C(C)\C)C)([H])[C@]2(CC[C@@H]3O)C

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 10 mg/mL (22.49 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2486 mL	11.2428 mL	22.4856 mL
5 mM	0.4497 mL	2.2486 mL	4.4971 mL
10 mM	0.2249 mL	1.1243 mL	2.2486 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.81%

Select Batch:

Data Sheet (279 KB) SDS (252 KB)

COA (248 KB) HNMR (285 KB) RP-HPLC (244 KB)

Handling Instructions (2659 KB)

References

[1]. Lee J, et al. Dammarenediol II enhances etoposide-induced apoptosis by targeting O-GlcNAc transferase and Akt/GSK3β/mTOR signaling in liver cancer. Mol Oncol. Published online December 30, 2025. [Content Brief]

[2]. Kim SH, et al. Dammarenediol-II Prevents VEGF-Mediated Microvascular Permeability in Diabetic Mice. Phytother Res. 2015;29(12):1910-1916. [Content Brief]

[3]. ERNESTO FATCORUSS, et al.
DAMMARANE TRITERPENES FROM THE RESIN OF BOSWELLIA FREERANA. Phytochemustry, Vol 24, No 5, pp 1035-1036, 1985.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2486 mL	11.2428 mL	22.4856 mL	56.2139 mL
	5 mM	0.4497 mL	2.2486 mL	4.4971 mL	11.2428 mL
	10 mM	0.2249 mL	1.1243 mL	2.2486 mL	5.6214 mL
	15 mM	0.1499 mL	0.7495 mL	1.4990 mL	3.7476 mL
	20 mM	0.1124 mL	0.5621 mL	1.1243 mL	2.8107 mL