DBCO-PEG4-Val-Ala-PAB
DBCO-PEG4-Val-Ala-PAB is a cleavable ADC linker. The Val-Ala linkers can be cleaved by Cathepsin B. The DBCO groups is commonly used for Click Chemistry reactions. PEG spacer improves the compound's aqueous solubility.
For research use only. We do not sell to patients.
- CAS No.: 2348405-92-3
- Formula: C45H57N5O10
- Molecular Weight:827.96
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
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Help & FAQs
Biological Activity
Description
Chemical Information
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CAS No. 2348405-92-3
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Molecular Weight 827.96
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Formula C45H57N5O10
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SMILES
CC([C@@H](C(N[C@H](C(NC1=CC=C(C=C1)CO)=O)C)=O)NC(CCOCCOCCOCCOCCNC(CCC(N2CC3=C(C#CC4=C2C=CC=C4)C=CC=C3)=O)=O)=O)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
DBCO-PEG4-Val-Ala-PAB Related Classifications
HY-W591373 Related Classifications
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