NUV-244

NUV-244 (1 μM; 24 h) significantly reduces the levels of PNPLA3^I148M on lipid droplets and restores normal lipid droplet morphology in Huh7 cells stably expressing PNPLA3 I148M‑tagRFP^[1].
NUV-244 (24 h) significantly reduces the levels of lipid droplet-associated untagged PNPLA3 I148M and untagged wild-type PNPLA3 in Huh7 cells, indicating that its activity does not depend on the presence of the tagRFP fusion tag^[1].
NUV-244 (24 h) exhibits activity in multiple liver-derived cell lines, including hepatocellular carcinoma cells and hepatic stellate cells, and retains equivalent potency in cells treated with Oleic acid (HY-N1446) to induce lipid droplet accumulation^[1].
NUV-244 (24 h) effectively degrades total intracellular PNPLA3^I148M and total PNPLA3 wild-type protein in viable Huh7 cells, confirming that the observed reduction of PNPLA3 signal at lipid droplets corresponds to complete degradation of this protein^[1].
NUV-244 (1-5 μM; 0-20 h) triggers a rapid reduction of PNPLA3^I148M on lipid droplets in Huh7 cells within 2 hours of administration, with the reduction approaching half of the maximum value^[1].
NUV-244 (25 μM) directly and selectively binds to purified PNPLA3 WT and PNPLA3 I148M proteins in a cell-free assay, and no interaction with PNPLA2 is detected^[1].
NUV-244 (24 h) effectively reduces the PNPLA3^I148M protein level in differentiated adipocytes, with an EC₅₀ of 2.02 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.