Deoxyribonuclease II
Based on 2 publication(s) in Google Scholar
Deoxyribonuclease II (DNase II) is an endonuclease that hydrolyzes the phosphodiester bonds of deoxyribonucleotides in native and denatured DNA, producing 3' phosphate and 5'-hydroxyl termini. Deoxyribonuclease II works best at acidic pH and is commonly used in biochemical research.
For research use only. We do not sell to patients.
- CAS No.: 9025-64-3
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Deoxyribonuclease II
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Biological Activity
Description
In Vitro
Product Information
Molecular Weight: 38.0 kDa
Optimal pH: 4.5-5.0
Activators: Cysteine, EDTA
Inhibitors: Actinomycin D, iodoacetate, N-bromosuccinimide, H2O2, divalent cations (such as zinc and copper), sulfate, NaCl (above 250 mM), DNA (above 4 mg/mL)
Instructions
Soluble in pure water (1 mg/mL), fresh preparation is recommended. Or dissolve in buffer according to specific experimental references.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
EC Number
3.1.22.1
Enzyme Activity
≥800 U/mg soild
Unit Definition
One unit is defined as the amount of enzyme that can causes an increase in absorbance at 260 nm of 0.001 per minute at 25°C, pH 4.6.
Chemical Information
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CAS No. 9025-64-3
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Appearance Solid
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Color White to light yellow
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SMILES
[Deoxyribonuclease II]
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Synonyms
DNase II
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (2)
Solvent & Solubility
In Vitro:
H2O : ≥ 5 mg/mL
* "≥" means soluble, but saturation unknown.
Purity & Documentation
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Data Sheet (267 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Deoxyribonuclease II Related Classifications
HY-P2863 Related Classifications
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