Deoxyribonuclease II
Based on 2 publication(s) in Google Scholar
Deoxyribonuclease II (DNase II) is an endonuclease that hydrolyzes the phosphodiester bonds of deoxyribonucleotides in native and denatured DNA, producing 3' phosphate and 5'-hydroxyl termini. Deoxyribonuclease II works best at acidic pH and is commonly used in biochemical research.
For research use only. We do not sell to patients.
- CAS No.: 9025-64-3
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications Citing Use of MedChemExpress (MCE) Deoxyribonuclease II
More
Biological Activity
Product Information
Molecular Weight: 38.0 kDa
Optimal pH: 4.5-5.0
Activators: Cysteine, EDTA
Inhibitors: Actinomycin D, iodoacetate, N-bromosuccinimide, H2O2, divalent cations (such as zinc and copper), sulfate, NaCl (above 250 mM), DNA (above 4 mg/mL)
Instructions
Soluble in pure water (1 mg/mL), fresh preparation is recommended. Or dissolve in buffer according to specific experimental references.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
3.1.22.1
≥800 U/mg soild
One unit is defined as the amount of enzyme that can causes an increase in absorbance at 260 nm of 0.001 per minute at 25°C, pH 4.6.
Chemical Information
-
CAS No. 9025-64-3
-
Appearance Solid
-
Color White to light yellow
-
SMILES
[Deoxyribonuclease II]
-
Synonyms
DNase II
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (2)
Solvent & Solubility
H2O : ≥ 5 mg/mL
* "≥" means soluble, but saturation unknown.
Purity & Documentation
-
Data Sheet (267 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)