Desaspidin
Desaspidin is an oxidative phosphorylation inhibitor and photophosphorylation inhibitor. Desaspidin uncouples mitochondrial oxidative phosphorylation, multiple chloroplast photophosphorylation pathways, and ATP synthesis linked to non-cyclic NADP reduction. Desaspidin can be used for the research of anthelmintic agent.
For research use only. We do not sell to patients.
- CAS No.: 114-43-2
- Formula: C24H30O8
- Molecular Weight:446.49
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Desaspidin (0.5-2.0 μM; 2 min) uncouples photophosphorylation in isolated spinach chloroplasts, with an IC50 of ~0.5 μM for XE accumulation, reducing XE half-life and ATP yield in a concentration-dependent manner[1].
Desaspidin (1.1 μM; 0.25-20 min) inhibits FMN-catalysed photophosphorylation in isolated spinach chloroplasts completely at short illumination times[1].
Desaspidin (0.1-5 μM) uncouples ferredoxin-catalysed cyclic photophosphorylation in isolated spinach chloroplasts with an IC50 of ~0.2 μM[1].
Desaspidin (100 nM-10 μM) acts as a potent, redox-dependent uncoupler of photophosphorylation in swiss chard, spinach, or lettuce chloroplasts[2].
Desaspidin (5 μM) initially uncouples FMN-driven photophosphorylation in swiss chard, spinach, or lettuce chloroplasts, but its inhibitory activity is lost over time due to photodestruction, with resistance reduced at lower chlorophyll concentrations[2].
Desaspidin (0.5 μM; 10 minutes) strongly inhibits both cyclic and noncyclic (reduced dichlorophenol indophenol-to-TPN) photophosphorylation[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 114-43-2
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Molecular Weight 446.49
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Formula C24H30O8
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SMILES
O=C1C(C(CCC)=O)=C(C(C)(C)C(O)=C1CC2=C(C(C(CCC)=O)=C(C=C2OC)O)O)O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Kinase activity and phosphorylation assays
Kinase activity assays measure the ability of kinases to transfer phosphate groups from ATP to specific substrates, while phosphorylation assays detect the presence and levels of phosphorylated proteins. Common methods include radiolabeled ATP incorporation (e. g. ,), ADP release detection via bioluminescence (e. g. ,[3]), enzyme-linked immunosorbent assays (ELISA) for phospho-specific epitopes (e. g. ,[6]), and microtiter-based formats for high-throughput screening (e. g. ,[8]). The ADP-Glo assay quantifies kinase activity by measuring ADP produced during phosphorylation using a luciferase-based system. Radiometric assays involve autoradiography or scintillation counting after incorporation of 32P-labeled ATP into substrate proteins. ELISA-based approaches rely on phospho-specific antibodies to detect activated kinases in cell lysates or purified samples.
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Western Blot
Western blotting (WB) is a commonly used experimental method in molecular biology, biochemistry, and immunogenetics for identifying and quantifying target proteins. It combines gel electrophoresis with immunoassay, enabling researchers to analyze protein expression, post-translational modifications, and molecular weight.
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Protocol for Kinase activity and phosphorylation assays
Kinase activity assays measure transfer of phosphate from ATP to a protein or peptide substrate, generating phosphorylated substrate, ADP, or incorporated radiolabeled phosphate as the readout; phosphorylation assays measure site-specific phosphorylation in cells or tissues as a proxy for kinase-pathway activation, inhibition, or substrate regulation. Phosphorylation can be detected by phospho-specific Western blot, immunoprecipitation kinase assay, phospho-immunofluorescence, phospho-flow cytometry, luminescent ADP detection, radiolabeled ATP incorporation, or reporter-based pathway assays, and these readouts can be applied to cancer cells, primary neurons, mouse tumors, organoids, inflammatory macrophages, ferroptosis studies, and mitophagy studies when the kinase target is biologically relevant.
Purity & Documentation
References
[1]. Hind G. Effect of desaspidin on photosynthetic phosphorylation and related processes. Nature. 1966 May 14;210(5037):703-8. [Content Brief]
[2]. Gromet-Elhanan Z, et al. Desaspidin: a nonspecific uncoupler of photophosphorylation. Plant Physiol. 1966 Sep;41(7):1231-6. [Content Brief]
[3]. Gromet-Elhanan Z, et al. Effect of desaspidin on photosynthetic phosphorylation. Plant Physiol. 1965 Nov;40(6):1060-5. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Desaspidin Related Classifications
HY-183972 Related Classifications
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