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Cat. No.: HY-185904

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Desthiobiotin-FP

Desthiobiotin-FP is a covalent activity-based probe used for labeling, detecting and enriching active serine hydrolases in complex protein samples. Desthiobiotin-FP combines a fluorophosphonate (FP) reactive group with a desthiobiotin tag for affinity pull-down. Desthiobiotin-FP enables activity profiling of serine- and cysteine-based enzymes.

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CAS No.: 1811556-65-6
Formule: C₂₂H₄₃FN₃O₄P
Masse moléculaire:463.57

Stockage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Desthiobiotin-FP Chemical Structure

CAS No.: 1811556-65-6

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- In Vitro
Chemical Information
Protocole (1)
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
Bioz

Activité biologique

Description

In Vitro

Desthiobiotin-FP (2 μM; 90 min) labels 18 serine and cysteine enzymes involved in lipid metabolism and cell wall biosynthesis in Mycobacterium tuberculosis mc²6230 lysates^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Chemical Information

CAS No. 1811556-65-6
Masse moléculaire 463.57
Formule C₂₂H₄₃FN₃O₄P
SMILES

CCOP(CCCCCCCCCCNC(CCCCC[C@H]1NC(N[C@H]1C)=O)=O)(F)=O
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocole

All Molecular Biology

Pull-down
The pull-down assay is an in vitro technique used to detect physical interactions between two or more proteins and an invaluable tool for confirming a predicted protein-protein interaction or identifying novel interacting partners. This method typically involves the use of affinity purification with various wash and elution steps.

Pureté et documentation

Instruction de manipulation (2659 KB)

Références

[1]. Nguyen PC, et al. Oxadiazolone derivatives, new promising multi-target inhibitors against M. tuberculosis. Bioorganic chemistry. 2018 Dec;81:414-424. [Content Brief]

Calculators

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

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100 mg

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