Dihydrorhodamine 6G
Based on 1 Customer Validation
Dihydrorhodamine 6G (DHR 6G) is the reduced form of Rhodamine 6G, which is used as fluorescent mitochondrial dye. It is nonfluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 6G that accumulates in mitochondrial membranes. Dihydrorhodamine 6G is useful for detecting reactive oxygen species (ROS) including superoxide.
For research use only. We do not sell to patients.
- Purity: 98.17%
- CAS No.: 217176-83-5
- Formula: C28H32N2O3
- Molecular Weight:444.57
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
Biological Activity
Chemical Information
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CAS No. 217176-83-5
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Appearance Solid
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Molecular Weight 444.57
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Formula C28H32N2O3
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Color Pale pink to pink
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SMILES
O=C(OCC)C1=CC=CC=C1C2C3=C(OC4=C2C=C(C)C(NCC)=C4)C=C(NCC)C(C)=C3
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Synonyms
DHR 6G
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 50 mg/mL (112.47 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2494 mL | 11.2468 mL | 22.4936 mL | 56.2341 mL |
| 5 mM | 0.4499 mL | 2.2494 mL | 4.4987 mL | 11.2468 mL | |
| 10 mM | 0.2249 mL | 1.1247 mL | 2.2494 mL | 5.6234 mL | |
| 15 mM | 0.1500 mL | 0.7498 mL | 1.4996 mL | 3.7489 mL | |
| 20 mM | 0.1125 mL | 0.5623 mL | 1.1247 mL | 2.8117 mL | |
| 25 mM | 0.0900 mL | 0.4499 mL | 0.8997 mL | 2.2494 mL | |
| 30 mM | 0.0750 mL | 0.3749 mL | 0.7498 mL | 1.8745 mL | |
| 40 mM | 0.0562 mL | 0.2812 mL | 0.5623 mL | 1.4059 mL | |
| 50 mM | 0.0450 mL | 0.2249 mL | 0.4499 mL | 1.1247 mL | |
| 60 mM | 0.0375 mL | 0.1874 mL | 0.3749 mL | 0.9372 mL | |
| 80 mM | 0.0281 mL | 0.1406 mL | 0.2812 mL | 0.7029 mL | |
| 100 mM | 0.0225 mL | 0.1125 mL | 0.2249 mL | 0.5623 mL |