DOTA-NHS-ester
Based on 1 publication(s) in Google Scholar
DOTA-NHS-ester is a bifunctional reagent containing a DOTA chelating group and an NHS ester. DOTA-NHS-ester can be conjugated to proteins/antibodies/HSA via primary amino groups for radiolabeling with radiometals, and when combined with 16-amino-1-hexadecanethiol hydrochloride, it enables the construction of self-assembled monolayers on gold surfaces for lectin microarrays. DOTA-NHS-ester can be used to prepare DOTA-HSA-Z (HER2:342) imaging probes, as well as to construct Trop2-targeted radiotherapeutic and diagnostic probes for pancreatic cancer.
For research use only. We do not sell to patients.
- Purity : 95.0%
- CAS No.: 170908-81-3
- Formula: C20H31N5O10
- Molecular Weight:501.49
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) DOTA-NHS-ester
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Customer Validation & Images
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In Vivo Efficacy Study
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In Vivo Efficacy Study
Biological Activity
Description
In Vitro
DOTA-NHS ester (3.78 mg; 2 h) successfully conjugates to HSA, yielding a DOTA-HSA bioconjugate with approximately two DOTA molecules per HSA molecule[1].
DOTA-NHS-ester (10 mM; overnight) in combination with 16-amino-1-hexadecanethiol hydrochloride successfully modifies gold chip surfaces, producing a rough surface topography and characteristic infrared absorption peaks consistent with covalent coupling of the reagent[2].
DOTA-NHS-ester (5-20 mM; 2 h) modification concentration on gold biochips positively correlates with IgG protein binding capacity, with 10 mM selected as the optimal working concentration balancing performance and reagent efficiency[2].
DOTA-NHS-ester (10 mM; overnight) modification of gold biochips proceeds effectively at both 25 °C and 37 °C, with 25 °C selected as the optimal working temperature for practical convenience[2].
DOTA-NHS-ester (10 mM; overnight) modified gold biochips support specific, concentration-dependent binding of ConA lectin and its corresponding antibody, validating the platform's suitability for lectin-based assays[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
Chemical Information
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CAS No. 170908-81-3
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Appearance Solid
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Molecular Weight 501.49
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Formula C20H31N5O10
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Color White to off-white
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SMILES
O=C(ON1C(CCC1=O)=O)CN2CCN(CC(O)=O)CCN(CC(O)=O)CCN(CC(O)=O)CC2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
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DOTA-NHS-ester purchased from MedChemExpress. Usage Cited in: Adv Nanobiomed Res. 2023 Sep 3.
PSP–NH2 was incubated with DOTA–NHS–ester for 1 h and conjugated with mPEG–NHS. Then, the particles were mixed with 64Cu (OAc)2 at 50 ℃ for 1 h to obtain the 64Cu-labelled PSP. PSP@Ab@PEG exhibited increased blood circulation in BALB/c mice with blood clearance half-life periods found to be 12 min.
DOTA-NHS-ester purchased from MedChemExpress. Usage Cited in: Adv Nanobiomed Res. 2023 Sep 3.
PSP–NH2 was incubated with DOTA–NHS–ester for 1 h and conjugated with mPEG–NHS. Then, the particles were mixed with 64Cu (OAc)2 at 50 ℃ for 1 h to obtain the 64Cu-labelled PSP. More PSP–NH2 accumulated in the liver site while a lot more PSP@Ab@PEG accumulated in the spleen.
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (99.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.99 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.99 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Hoppmann S, et al. Radiolabeled affibody-albumin bioconjugates for HER2-positive cancer targeting. Bioconjugate chemistry. 2011 Mar 16;22(3):413-21. [Content Brief]
[3]. Li C, et al. Theranostic application of Cu/Lu-labeled anti-Trop2 monoclonal antibody in pancreatic cancer tumor models. European journal of nuclear medicine and molecular imaging. 2022 Dec;50(1):168-183. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9941 mL | 9.9703 mL | 19.9406 mL | 49.8514 mL |
| 5 mM | 0.3988 mL | 1.9941 mL | 3.9881 mL | 9.9703 mL | |
| 10 mM | 0.1994 mL | 0.9970 mL | 1.9941 mL | 4.9851 mL | |
| 15 mM | 0.1329 mL | 0.6647 mL | 1.3294 mL | 3.3234 mL | |
| 20 mM | 0.0997 mL | 0.4985 mL | 0.9970 mL | 2.4926 mL | |
| 25 mM | 0.0798 mL | 0.3988 mL | 0.7976 mL | 1.9941 mL | |
| 30 mM | 0.0665 mL | 0.3323 mL | 0.6647 mL | 1.6617 mL | |
| 40 mM | 0.0499 mL | 0.2493 mL | 0.4985 mL | 1.2463 mL | |
| 50 mM | 0.0399 mL | 0.1994 mL | 0.3988 mL | 0.9970 mL | |
| 60 mM | 0.0332 mL | 0.1662 mL | 0.3323 mL | 0.8309 mL | |
| 80 mM | 0.0249 mL | 0.1246 mL | 0.2493 mL | 0.6231 mL |
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