DSPE-PEG2000-Mannose 

DSPE-PEG 2000-Mannose is a mannose-containing lipid. DSPE-PEG 2000-Mannose is used to prepare mannose-conjugated Liposome (Man-lipo) for siRNA delivery. Mannose-modified liposomes encapsulating IDO siRNA (Man-lipo-siIDO) preferentially knock down IDO expression in the draining lymph nodes and spleens of melanoma-bearing mice. Man-lipo-siIDO delays the onset time of melanoma and reduces tumor volume^[1].

DSPE-PEG2000-Mannose (3:10:10:4 molar ratio with DOTAP, DPPC, cholesterol; 10 min hydration, 10 min post-extrusion incubation) was used to prepare mannose-conjugated liposomes (Man-lipo) for siRNA delivery, with formulation steps including lipid mixing, hydration with siRNA-protamine mixture, vortexing, sonication, extrusion, and post-extrusion incubation^[1].
DSPE-PEG2000-Mannose-formulated Man-lipo-siIDO (24 h post-transfection) significantly reduced IDO mRNA levels in mouse bone marrow-derived dendritic cells (DCs) compared to Man-lipo-siScramble control^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Treatment with DSPE-PEG2000-Mannose liposomes encapsulating IDO siRNA (100 μg; i.v.; once 2 days pre-tumor inoculation then once weekly thereafter) significantly prolongs tumor-free survival, reduces tumor burden, inhibits T-cell apoptosis, increases CD8⁺ T-cell populations in tumor-draining lymph nodes, reduces T regulatory cell levels, and enhances tumor antigen-specific T-cell proliferation in melanoma-bearing C57BL/6 mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2000 (Average)

1829524-73-3

Solid

White to off-white

CCCCCCCCCCCCCCCCCC(O[C@@H](COP(OCCNC(OCCOCCNC(NC1=CC=C(O[C@@H]2[C@@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O2)C=C1)=S)=O)(O)=O)COC(CCCCCCCCCCCCCCCCC)=O)=O.[n]

Room temperature in continental US; may vary elsewhere.

• [1]. Chen D, et al. Targeted siRNA silencing of indoleamine 2, 3-dioxygenase in antigen-presenting cells using mannose-conjugated liposomes: a novel strategy for treatment of melanoma. J Immunother. 2014;37(2):123-134.  [Content Brief]