Epimedonin H
Epimedonin H is a selective prenylated 2-phenoxychromone compound with no significant cytotoxicity against five human cancer cell lines, including HL-60 and SMMC-7721 (IC50 > 10 μM). Epimedonin H can be used in potential activity screening studies in the field of natural product chemistry. Epimedonin H can be extracted from the dried aerial parts of Epimedium brevicornum (a plant of the genus Epimedium in the Berberidaceae family).
For research use only. We do not sell to patients.
- CAS No.: 2222285-82-5
- Formula: C20H18O7
- Molecular Weight:370.35
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | GI |
50 %
Compound: 3
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Growth inhibition of human A549 cells at 40 uM after 48 hrs by MTS assay relative to control
Growth inhibition of human A549 cells at 40 uM after 48 hrs by MTS assay relative to control
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[PMID: 29338226] |
| A549 | IC50 |
>10 μM
Compound: 3
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Cytotoxicity against human A549 cells after 48 hrs by MTS assay
Cytotoxicity against human A549 cells after 48 hrs by MTS assay
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[PMID: 29338226] |
| HL-60 | IC50 |
>10 μM
Compound: 3
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Cytotoxicity against human HL60 cells after 48 hrs by MTS assay
Cytotoxicity against human HL60 cells after 48 hrs by MTS assay
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[PMID: 29338226] |
| MCF7 | IC50 |
>10 μM
Compound: 3
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Cytotoxicity against human MCF7 cells after 48 hrs by MTS assay
Cytotoxicity against human MCF7 cells after 48 hrs by MTS assay
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[PMID: 29338226] |
| SMMC-7721 | IC50 |
>10 μM
Compound: 3
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Cytotoxicity against human SMMC7721 cells after 48 hrs by MTS assay
Cytotoxicity against human SMMC7721 cells after 48 hrs by MTS assay
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[PMID: 29338226] |
| SW480 | IC50 |
>10 μM
Compound: 3
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Cytotoxicity against human SW480 cells after 48 hrs by MTS assay
Cytotoxicity against human SW480 cells after 48 hrs by MTS assay
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[PMID: 29338226] |
Chemical Information
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CAS No. 2222285-82-5
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Molecular Weight 370.35
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Formula C20H18O7
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SMILES
CC(C(O)CC1=C2C(C(C=C(O2)OC3=CC=C(C=C3)O)=O)=C(C=C1O)O)=C
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Epimedonin H Related Classifications
HY-N9674 Related Classifications
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